Anti-Bid antibody (ab2388)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Bid antibody
See all Bid primary antibodies -
DescriptionRabbit polyclonal to Bid
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Host speciesRabbit
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SpecificityThis antibody detects full length (22 kDa) Bid.
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Tested applicationsSuitable for: IHC-Fr, IP, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, surrounding amino acid 37 of Human Bid. (Peptide available as ab38270.)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS, pH 7.2 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab2388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use a concentration of 20 - 40 µg/ml. | |
| IP | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.Can be blocked with Bid peptide (ab38270). |
Target
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FunctionThe major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
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Tissue specificityIsoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
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DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
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Post-translational
modificationsTNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. -
Cellular localizationCytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
- Information by UniProt
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Database links
- Entrez Gene: 637 Human
- Omim: 601997 Human
- SwissProt: P55957 Human
- Unigene: 591054 Human
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Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist BID antibody
- BH3 interacting domain death agonist antibody
see all
Images
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Western blot - Anti-Bid antibody (ab2388)Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.
Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.
References
This product has been referenced in:
- Piao X et al. 1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway. Front Pharmacol 9:372 (2018). Read more (PubMed: 29725297) »
- Shinozaki-Ushiku A et al. Profiling of Virus-Encoded MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma and Their Roles in Gastric Carcinogenesis. J Virol 89:5581-91 (2015). Read more (PubMed: 25740983) »
