Anti-Bid antibody (ab2388)

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Western blot - Anti-Bid antibody (ab2388)

    Key features and details

    • Rabbit polyclonal to Bid
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-Bid antibody
      See all Bid primary antibodies

    • Description

      Rabbit polyclonal to Bid

    • Host species

      Rabbit

    • Specificity

      This antibody detects full length (22 kDa) Bid.

    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data

    • Immunogen

      Synthetic peptide, surrounding amino acid 37 of Human Bid. (Peptide available as ab38270.)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2388 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.

    Target

    • Function

      The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.

    • Tissue specificity

      Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

    • Domain

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

    • Post-translational
      modifications

      TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.

    • Cellular localization

      Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
    • Information by UniProt

    • Database links

      • Alternative names

        • Apoptic death agonist antibody
        • Apoptotic death agonist BID antibody
        • BH3 interacting domain death agonist antibody
        • BH3 interacting domain death agonist p11 antibody
        • BH3 interacting domain death agonist p13 antibody
        • BH3 interacting domain death agonist p15 antibody
        • BH3-interacting domain death agonist p11 antibody
        • BID antibody
        • BID isoform ES(1b) antibody
        • BID isoform L(2) antibody
        • BID isoform Si6 antibody
        • BID_HUMAN antibody
        • Desmocollin type 4 antibody
        • FP497 antibody
        • Human BID coding sequence antibody
        • MGC15319 antibody
        • MGC42355 antibody
        • p11 BID antibody
        • p13 BID antibody
        • p15 BID antibody
        • p22 BID antibody
        see all

      Images

      • Western blot - Anti-Bid antibody (ab2388)
        Western blot - Anti-Bid antibody (ab2388)
        Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours. Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.

      References (3)

      Publishing research using ab2388? Please let us know so that we can cite the reference in this datasheet.

      ab2388 has been referenced in 3 publications.

      • Subhawa S  et al. The Effects of Houttuynia cordata Thunb and Piper ribesioides Wall Extracts on Breast Carcinoma Cell Proliferation, Migration, Invasion and Apoptosis. Molecules 25:N/A (2020). PubMed: 32155880
      • Piao X  et al. 1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway. Front Pharmacol 9:372 (2018). PubMed: 29725297
      • Shinozaki-Ushiku A  et al. Profiling of Virus-Encoded MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma and Their Roles in Gastric Carcinogenesis. J Virol 89:5581-91 (2015). PubMed: 25740983

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      I want to do immunostaining for cleaved BID (p15), whether this product can detect cleaved BID (p15)

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      Answer

      Thank you for your enquiry. Yes, this antibody detects both full length (22 kDa) and cleaved fragments (15 kDa) of Bid. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      Question

      Can you send me an image? I am having a hard time with seeing multiple bands.

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      Answer

      Thank you for your enquiry. I have added an image of a Western blot using this antibody onto the datasheet. For your convenience, I have included a link to the datasheet at the bottom of this email. If you continue to have a problem with this antibody, please fill out the questionnaire located at the following link, and we will try to help you troubleshoot the problem: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=2388&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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      Question

      I want to know one of your primary antibody's immunogen. It will help me to know whether it is possible to react with chick. Its catalog number is ab2388. Thanks a lot!

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      Answer

      The immunogen is a synthetic peptide containing 15 amino acids around amino acids 40 of human Bid.

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