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    bid-antibody-ab2388.pdf

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Cell Biology Apoptosis Intracellular Bcl2 Family
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Anti-Bid antibody (ab2388)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (3)

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Western blot - Anti-Bid antibody (ab2388)

    Key features and details

    • Rabbit polyclonal to Bid
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human Bid protein (ab85155)

    View more associated products

    Overview

    • Product name

      Anti-Bid antibody
      See all Bid primary antibodies
    • Description

      Rabbit polyclonal to Bid
    • Host species

      Rabbit
    • Specificity

      This antibody detects full length (22 kDa) Bid.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide, surrounding amino acid 37 of Human Bid. (Peptide available as ab38270.)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.20
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: PBS, 30% Glycerol, 0.5% BSA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Bcl2 Family
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Bcl 2 family
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Apoptosis Markers
      • Bcl 2 family
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Bid protein (ab85155)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2388 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.

    Target

    • Function

      The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
    • Tissue specificity

      Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
    • Domain

      Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • Post-translational
      modifications

      TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
      p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
    • Cellular localization

      Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
    • Target information above from: UniProt accession P55957 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 637 Human
      • Omim: 601997 Human
      • SwissProt: P55957 Human
      • Unigene: 591054 Human
      • Alternative names

        • Apoptic death agonist antibody
        • Apoptotic death agonist BID antibody
        • BH3 interacting domain death agonist antibody
        • BH3 interacting domain death agonist p11 antibody
        • BH3 interacting domain death agonist p13 antibody
        • BH3 interacting domain death agonist p15 antibody
        • BH3-interacting domain death agonist p11 antibody
        • BID antibody
        • BID isoform ES(1b) antibody
        • BID isoform L(2) antibody
        • BID isoform Si6 antibody
        • BID_HUMAN antibody
        • Desmocollin type 4 antibody
        • FP497 antibody
        • Human BID coding sequence antibody
        • MGC15319 antibody
        • MGC42355 antibody
        • p11 BID antibody
        • p13 BID antibody
        • p15 BID antibody
        • p22 BID antibody
        see all

      Images

      • Western blot - Anti-Bid antibody (ab2388)
        Western blot - Anti-Bid antibody (ab2388)
        Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours. Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab2388? Please let us know so that we can cite the reference in this datasheet.

      ab2388 has been referenced in 3 publications.

      • Subhawa S  et al. The Effects of Houttuynia cordata Thunb and Piper ribesioides Wall Extracts on Breast Carcinoma Cell Proliferation, Migration, Invasion and Apoptosis. Molecules 25:N/A (2020). PubMed: 32155880
      • Piao X  et al. 1-Deoxynojirimycin (DNJ) Ameliorates Indomethacin-Induced Gastric Ulcer in Mice by Affecting NF-kappaB Signaling Pathway. Front Pharmacol 9:372 (2018). PubMed: 29725297
      • Shinozaki-Ushiku A  et al. Profiling of Virus-Encoded MicroRNAs in Epstein-Barr Virus-Associated Gastric Carcinoma and Their Roles in Gastric Carcinogenesis. J Virol 89:5581-91 (2015). PubMed: 25740983

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      I want to do immunostaining for cleaved BID (p15), whether this product can detect cleaved BID (p15)

      Read More

      Abcam community

      Verified customer

      Asked on Apr 19 2006

      Answer

      Thank you for your enquiry. Yes, this antibody detects both full length (22 kDa) and cleaved fragments (15 kDa) of Bid. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Apr 21 2006

      Question

      Can you send me an image? I am having a hard time with seeing multiple bands.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 19 2006

      Answer

      Thank you for your enquiry. I have added an image of a Western blot using this antibody onto the datasheet. For your convenience, I have included a link to the datasheet at the bottom of this email. If you continue to have a problem with this antibody, please fill out the questionnaire located at the following link, and we will try to help you troubleshoot the problem: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=2388&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jan 19 2006

      Question

      I want to know one of your primary antibody's immunogen. It will help me to know whether it is possible to react with chick. Its catalog number is ab2388. Thanks a lot!

      Read More

      Abcam community

      Verified customer

      Asked on Nov 30 2004

      Answer

      The immunogen is a synthetic peptide containing 15 amino acids around amino acids 40 of human Bid.

      Read More

      Abcam Scientific Support

      Answered on Dec 09 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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