Anti-Bid antibody (ab2561)

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Overview

  • Product name
    Anti-Bid antibody
    See all Bid primary antibodies
  • Description
    Rabbit polyclonal to Bid
  • Host species
    Rabbit
  • Specificity
    This antibody detects full length (22 kDa) mouse Bid.
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide surrounding amino acid 45 of Mouse Bid.

Properties

Applications

Our Abpromise guarantee covers the use of ab2561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml.

Target

  • Function
    The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
  • Tissue specificity
    Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
  • Domain
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Post-translational
    modifications
    TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
  • Cellular localization
    Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
  • Information by UniProt
  • Database links
    • Alternative names
      • Apoptic death agonist antibody
      • Apoptotic death agonist BID antibody
      • BH3 interacting domain death agonist antibody
      • BH3 interacting domain death agonist p11 antibody
      • BH3 interacting domain death agonist p13 antibody
      • BH3 interacting domain death agonist p15 antibody
      • BH3-interacting domain death agonist p11 antibody
      • BID antibody
      • BID isoform ES(1b) antibody
      • BID isoform L(2) antibody
      • BID isoform Si6 antibody
      • BID_HUMAN antibody
      • Desmocollin type 4 antibody
      • FP497 antibody
      • Human BID coding sequence antibody
      • MGC15319 antibody
      • MGC42355 antibody
      • p11 BID antibody
      • p13 BID antibody
      • p15 BID antibody
      • p22 BID antibody
      see all

    References

    This product has been referenced in:
    See all 3 Publications for this product

    Publishing research using ab2561? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Question

    I am seeing a 37kD band on my Western blot. Do you have any ideas what this may be?

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    Answer

    Thank you for your enquiry. I have contacted the scientists that tested this antibody, and they stated that they only observe one single band at 22kDa with the antibody. I am sorry that we don't have any further insight as to what the 37kD band is that you are seeing. Please do not hesitate to contact us if you need anything further.

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    Question

    Thank you for your prompt answer. Could you find out if the peptide the company use as immunogen is in the portion where the proteins have 80 % homology with the rat or in the 20% where they are different. We already bought the antibody.I'm changing conditions in the western blot to see if I have the protein. We are working with a luteal cell line derived from rat.

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    Answer

    The originator of ab2561 was only able to inform me that the immunogen used was a synthetic peptide surrounding the cleavage site of mouse Bid.

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    Question

    I'd like to know if this antibody can react against rat.

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    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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    Question

    Can you tell us please what positive controls were used in the Western blot and any other details from the testing?

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    Answer

    L929 cell lysate can be used as a positive control for the anti-Bid antibody.

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