Overview

  • Product name

    Anti-Bid antibody - Carboxyterminal end
    See all Bid primary antibodies
  • Description

    Rabbit polyclonal to Bid - Carboxyterminal end
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with Bid
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the carboxy-terminus of human Bid

  • Positive control

    • Mouse lung cell lysates and mouse lung tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 2 µg/ml.

Target

  • Function

    The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
  • Tissue specificity

    Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Post-translational
    modifications

    TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
  • Cellular localization

    Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptic death agonist antibody
    • Apoptotic death agonist BID antibody
    • BH3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist p11 antibody
    • BH3 interacting domain death agonist p13 antibody
    • BH3 interacting domain death agonist p15 antibody
    • BH3-interacting domain death agonist p11 antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • BID_HUMAN antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p11 BID antibody
    • p13 BID antibody
    • p15 BID antibody
    • p22 BID antibody
    see all

Images

  • Lane 1 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 0.5 µg/ml
    Lane 2 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1 µg/ml
    Lane 3 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 2 µg/ml

    All lanes : Mouse lung cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti rabbit IgG secondary antibody

    Predicted band size: 22 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical staining of mouse lung tissue using ab62469 antibody at 2 µg/ml.
  • Immunofluorescence of Bid in Mouse Lung cells using ab62469 at 10 ug/ml.

  • All lanes : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1/1000 dilution

    Lane 1 : HCT116 colon cancer cell line treated with DMSO (control)
    Lane 2 : HCT116 colon cancer cell line treated with 2 µM SAHA for 24 hrs

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa


    Exposure time: 10 seconds

    See Abreview

References

This product has been referenced in:

  • Kinsella S  et al. Increased A20-E3 ubiquitin ligase interactions in bid-deficient glia attenuate TLR3- and TLR4-induced inflammation. J Neuroinflammation 15:130 (2018). Read more (PubMed: 29720226) »
  • Sula Karreci E  et al. Human regulatory T cells undergo self-inflicted damage via granzyme pathways upon activation. JCI Insight 2:N/A (2017). WB . Read more (PubMed: 29093262) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF cell line)
Loading amount
30 µg
Specification
MEF cell line
Treatment
DMSO control and 2 µM SAHA for 24 hrs
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Christian Marx

Verified customer

Submitted Feb 21 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount
30 µg
Specification
HCT116 colon cancer cell line
Treatment
DMSO control and 2 µM SAHA for 24 hrs
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Christian Marx

Verified customer

Submitted Feb 21 2013

Answer

Vielen Dank für Ihren Anruf.

Ich habe diesen Fall mit dem Labor besprochen. Leider konntenwirauch zusammenkeine Erklärung und somit Lösung dieses Problems mit diesem Röhrchen des ab62469 finden. Auch das Labor hat keine negatives Feedback zu diesem Antikörper bekommen. Ich denke deshalb, dass das Röhrchen, welches Sie bekommen haben, beschädigt sein muss - entweder durch den Transport oder bei der Lagerung. Ich werde Ihnen deshalb gerne dieses Röhrchen ersetzen, entweder mit einem neuem Röhrchen von ab62469, mit einer Rückzahlung oder mit einem Rährchen eines anderen Antikörpers.

Wir haben auch andere Antikörper gegen BID, welche das BID im C terminalen Bereich erkennen:

Der ab31273 wurde gegen ein Peptide des C terminus, von Aminosäure 184 - 195, gemacht. Dieser Antikörper wurde auch gegen das humane Protein und im Western blot getestet. https://www.abcam.com/index.html?datasheet=31273 (or use the following: https://www.abcam.com/index.html?datasheet=31273).

Der ab14437 wurde gegen ein Peptide in der C terminalen Region gemacht, ein Peptide von innerhalb der Sequenzvon Aminosäure120 -180. Dieser Antikörper wurde auch gegen das humane Protein und im Western blot getestet. https://www.abcam.com/index.html?datasheet=14437 (or use the following: https://www.abcam.com/index.html?datasheet=14437).

Bitte lassen Sie mich wissen, wie Sie vorgehen möchten oder ob Sie fragen haben. Ich freue mich wieder von Ihnen zu hören.

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Question
Answer

Vielen Dank für Ihren Anruf.

Leider kann ich Ihnen die genaue Immunogensequenez nicht geben, ich kann Ihnen jedoch bestätigen, dass es eine Sequenz vom C Terminus ist, inzwischen den Aminosäuren 181 - 241.

Ich hoffe diese Information ist Ihnen hilfreich. Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen haben.

Ich wünsche Ihnen ein schönes Wochenende!

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