• Product name

    Anti-Bid Cleavage Site antibody
    See all Bid Cleavage Site primary antibodies
  • Description

    Rabbit polyclonal to Bid Cleavage Site
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).

  • Positive control

    • TNF alpha treated L929 cells.
  • General notes

    BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.



Our Abpromise guarantee covers the use of ab10640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 15 kDa.


  • Relevance

    Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
  • Database links

  • Alternative names

    • Apoptic death agonist antibody
    • BH 3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p22 BID antibody
    see all


  • Mouse L929 cells were treated with 5 µg/mL actinomycin plus 2.5 ng/mL, 5 ng/mL and 10 ng/mL mouse TNF alpha for 5 hours (Lane 3, 4 and 5). Untreated L929 cells (Lane 1) and cells treated with actinomycin alone (Lane 2) were used as controls. Proteins were resolved from the cells by SDS-PAGE. The proteins were transferred to PVDF membranes. The membranes were incubated with the anti-mouse full length BID antibody (left panel) and ab10640 (right panel) at 1 µg/mL. The signal was detected using a goat F(ab’)2 anti-rabbit IgG alkaline phosphatase antibody at a 1/5000 dilution and the membrane was incubated with CDP-substrate followed by chemiluminescence. The data show that ab10640 only recognizes the cleaved mouse BID and not full length (Right panel). The full length BID was revealed using an anti-mouse full length BID antibody (Left panel). Mouse L929 cells were treated with 5 µg/mL actinomycin plus 2.5 ng/mL, 5 ng/mL and 10


This product has been referenced in:

  • Yen JH  et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
  • Liao Y  et al. Endoplasmic Reticulum Stress Induces Macrophages to Produce IL-1ß During Mycobacterium bovis Infection via a Positive Feedback Loop Between Mitochondrial Damage and Inflammasome Activation. Front Immunol 10:268 (2019). Read more (PubMed: 30846986) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Human Cell lysate - whole cell (Breast)
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C

Abcam user community

Verified customer

Submitted Mar 18 2013


Thank you for your enquiry. The antiserum was produced against a chemically synthesized peptide derived from the N-terminus of cleavage site of mouse BID. Ab10640 recognizes the epitope which is created when BID is cleaved by caspase 8.Caspase -8 cleaves between amino acid residues 59 and 60 of the mouse BID protein. This product has not been tested for use against rat and so I am unable to comment whether the product will detect the rat version of the target; however rat BID is only 43% homologous with mouse BID. For your convenience the mouse Swiss Prot entry for mouse BID is attached below: http://au.expasy.org/cgi-bin/niceprot.pl?P70444 If you have any further questions then please get back in touch with me.

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