Key features and details
- Rabbit polyclonal to Bid Cleavage Site
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-Bid Cleavage Site antibody
DescriptionRabbit polyclonal to Bid Cleavage Site
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Mouse Bid Cleavage Site (N terminal). Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).
Database link: P70444
- WB: 3T3-L1 cells extracts. ICC: A549 cells.
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from rabbit serum by epitope-specific affinity chromatography.
Primary antibody notesBH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 15 kDa.
1/1000. Predicted molecular weight: 15 kDa.
RelevanceBid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
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All lanes : Anti-Bid Cleavage Site antibody (ab10640) at 1/1000 dilution
Lane 1 : 3T3-L1 cells without BID
Lane 2 : 3T3-L1 cells with caspase-8 cleaved recombinant mouse BID
Lane 3 : 3T3-L1 cells with caspase-8 cleaved recombinant human BID
Predicted band size: 15 kDa
SDS-PAGE on a 4-20% Tris-glycine gel
A549 cells stained for BID cleave site (green) using ab10640 at 1/250 dilution in ICC/IF. It was followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control.
ab10640 has been referenced in 14 publications.
- Zhang Y et al. Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics. Nat Commun 11:5421 (2020). PubMed: 33110072
- Sheng H et al. Liriopesides B induces apoptosis and cell cycle arrest in human non-small cell lung cancer cells. Int J Mol Med 46:1039-1050 (2020). PubMed: 32705266
- Chung TW et al. Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells. Onco Targets Ther 12:6119-6131 (2019). PubMed: 31496721
- Li X et al. A novel cell-penetrating peptide protects against neuron apoptosis after cerebral ischemia by inhibiting the nuclear translocation of annexin A1. Cell Death Differ 26:260-275 (2019). PubMed: 29769639
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497