Anti-Bif-1 antibody (ab154236)
Key features and details
- Rabbit polyclonal to Bif-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Bif-1 antibody
See all Bif-1 primary antibodies -
Description
Rabbit polyclonal to Bif-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Bif-1 aa 302-365 (C terminal).
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Positive control
- H1299, NIH 3T3, JC, BCL1 whole cell lysates; U87 xenograft tissue; A431 cells.
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General notes
Previously labelled as Bif.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability. -
Tissue specificity
Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. -
Sequence similarities
Belongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain. -
Domain
An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
The SH3 domain is required and sufficient for the interaction with UVRAG. -
Post-translational
modificationsPhosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Mitochondrion outer membrane. Cytoplasmic vesicle, autophagosome membrane. Midbody. Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140). - Information by UniProt
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Database links
- Entrez Gene: 51100 Human
- Entrez Gene: 54673 Mouse
- Omim: 609287 Human
- SwissProt: Q9Y371 Human
- SwissProt: Q9JK48 Mouse
- Unigene: 136309 Human
- Unigene: 271775 Mouse
- Unigene: 440295 Mouse
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Alternative names
- Bax interacting factor 1 antibody
- Bax-interacting factor 1 antibody
- Bif 1 antibody
see all
Images
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Western blot - Anti-Bif-1 antibody (ab154236)Anti-Bif-1 antibody (ab154236) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 40 kDa
7.5% SDS PAGE -
Western blot - Anti-Bif-1 antibody (ab154236)All lanes : Anti-Bif-1 antibody (ab154236) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Bif-1 antibody (ab154236)Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Bif-1 with ab154236 at 1/200 dilution. Lower panel merged with DNA probe.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bif-1 antibody (ab154236)Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Bif-1 with ab154236 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154236 has not yet been referenced specifically in any publications.