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    bif-1-antibody-ab154236.pdf

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Cell Biology Apoptosis Mitochondrial
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Anti-Bif-1 antibody (ab154236)

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Western blot - Anti-Bif-1 antibody (ab154236)
  • Western blot - Anti-Bif-1 antibody (ab154236)
  • Immunocytochemistry/ Immunofluorescence - Anti-Bif-1 antibody (ab154236)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bif-1 antibody (ab154236)

Key features and details

  • Rabbit polyclonal to Bif-1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Bif-1 antibody
    See all Bif-1 primary antibodies
  • Description

    Rabbit polyclonal to Bif-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Bif-1 aa 302-365 (C terminal).

  • Positive control

    • H1299, NIH 3T3, JC, BCL1 whole cell lysates; U87 xenograft tissue; A431 cells.
  • General notes

    Previously labelled as Bif.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Bif-1 protein (ab137149)
  • Related Products

    • Recombinant Human Bif-1 protein (ab137149)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/1000.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability.
  • Tissue specificity

    Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Domain

    An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
    The SH3 domain is required and sufficient for the interaction with UVRAG.
  • Post-translational
    modifications

    Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Mitochondrion outer membrane. Cytoplasmic vesicle, autophagosome membrane. Midbody. Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140).
  • Target information above from: UniProt accession Q9Y371 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51100 Human
    • Entrez Gene: 54673 Mouse
    • Omim: 609287 Human
    • SwissProt: Q9Y371 Human
    • SwissProt: Q9JK48 Mouse
    • Unigene: 136309 Human
    • Unigene: 271775 Mouse
    • Unigene: 440295 Mouse
    • Alternative names

      • Bax interacting factor 1 antibody
      • Bax-interacting factor 1 antibody
      • Bif 1 antibody
      • Bif antibody
      • Bif-1 antibody
      • CGI 61 antibody
      • dJ612B15.2 antibody
      • Endophilin B1 antibody
      • Endophilin-B1 antibody
      • PPP1R70 antibody
      • Protein phosphatase 1 regulatory subunit 70 antibody
      • SH3 containing protein SH3GLB1 antibody
      • SH3 domain GRB2 like endophilin B1 antibody
      • SH3 domain-containing GRB2-like protein B1 antibody
      • Sh3glb1 antibody
      • SH3p13 antibody
      • SHLB1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Bif-1 antibody (ab154236)
      Western blot - Anti-Bif-1 antibody (ab154236)
      Anti-Bif-1 antibody (ab154236) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

      Predicted band size: 40 kDa



      7.5% SDS PAGE
    • Western blot - Anti-Bif-1 antibody (ab154236)
      Western blot - Anti-Bif-1 antibody (ab154236)
      All lanes : Anti-Bif-1 antibody (ab154236) at 1/1000 dilution

      Lane 1 : NIH 3T3 whole cell lysate
      Lane 2 : JC whole cell lysate
      Lane 3 : BCL1 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 40 kDa



      10% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Bif-1 antibody (ab154236)
      Immunocytochemistry/ Immunofluorescence - Anti-Bif-1 antibody (ab154236)

      Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Bif-1 with ab154236 at 1/200 dilution. Lower panel merged with DNA probe.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bif-1 antibody (ab154236)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bif-1 antibody (ab154236)

      Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Bif-1 with ab154236 at 1/500 dilution.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154236? Please let us know so that we can cite the reference in this datasheet.

    ab154236 has not yet been referenced specifically in any publications.

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