Overview

  • Product name

  • Description

    Rabbit polyclonal to Bif-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Bif-1 aa 302-365 (C terminal).

  • Positive control

    • H1299, NIH 3T3, JC, BCL1 whole cell lysates; U87 xenograft tissue; A431 cells.
  • General notes

    Previously labelled as Bif.

Properties

Applications

Our Abpromise guarantee covers the use of ab154236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability.
  • Tissue specificity

    Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Domain

    An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
    The SH3 domain is required and sufficient for the interaction with UVRAG.
  • Post-translational
    modifications

    Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Mitochondrion outer membrane. Cytoplasmic vesicle, autophagosome membrane. Midbody. Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140).
  • Information by UniProt
  • Database links

  • Alternative names

    • Bax interacting factor 1 antibody
    • Bax-interacting factor 1 antibody
    • Bif 1 antibody
    • Bif antibody
    • Bif-1 antibody
    • CGI 61 antibody
    • dJ612B15.2 antibody
    • Endophilin B1 antibody
    • Endophilin-B1 antibody
    • PPP1R70 antibody
    • Protein phosphatase 1 regulatory subunit 70 antibody
    • SH3 containing protein SH3GLB1 antibody
    • SH3 domain GRB2 like endophilin B1 antibody
    • SH3 domain-containing GRB2-like protein B1 antibody
    • Sh3glb1 antibody
    • SH3p13 antibody
    • SHLB1_HUMAN antibody
    see all

Images

  • Anti-Bif-1 antibody (ab154236) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    7.5% SDS PAGE
  • All lanes : Anti-Bif-1 antibody (ab154236) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling Bif-1 with ab154236 at 1/200 dilution. Lower panel merged with DNA probe.

  • Immunohistochemical analysis of paraffin-embedded U87 xenograft tissue labeling Bif-1 with ab154236 at 1/500 dilution.

References

ab154236 has not yet been referenced specifically in any publications.

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