Recombinant
RabMAb

Recombinant Anti-BIG-2 antibody [EPR8736] (ab131285)

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Overview

  • Product name

    Anti-BIG-2 antibody [EPR8736]
    See all BIG-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8736] to BIG-2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human BIG-2 aa 800-900.
    Database link: Q8IWV2

  • Positive control

    • Fetal brain lysate, Human cerebellum lysate. Human kidney tissue and Human brain tissue.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.

    • Tissue specificity

      Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex.

    • Involvement in disease

      A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. Contactin family.
      Contains 4 fibronectin type-III domains.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

    • Cellular localization

      Cell membrane. Secreted.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • AXCAM antibody
      • Axonal associated cell adhesion molecule antibody
      • BIG 2 antibody
      • BIG-2 antibody
      • Brain derived immunoglobulin superfamily protein 2 antibody
      • Brain-derived immunoglobulin superfamily protein 2 antibody
      • Cntn4 antibody
      • CNTN4_HUMAN antibody
      • CNTN4A antibody
      • contactin 4 antibody
      • Contactin-4 antibody
      • Neural cell adhesion protein BIG 2 antibody
      • SCA16 antibody
      see all

    Images

    • Western blot - Anti-BIG-2 antibody [EPR8736] (ab131285)
      Western blot - Anti-BIG-2 antibody [EPR8736] (ab131285)
      All lanes : Anti-BIG-2 antibody [EPR8736] (ab131285) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 113 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelled with ab131285 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab131285 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab131285 has not yet been referenced specifically in any publications.

    Publishing research using ab131285? Please let us know so that we can cite the reference in this datasheet.

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