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Neuroscience Cell Adhesion Proteins Membrane Proteins
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RecombinantRabMAb

Recombinant Anti-BIG-2 antibody [EPR8736] (ab131285)

  • Datasheet
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Western blot - Anti-BIG-2 antibody [EPR8736] (ab131285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
  • Anti-BIG-2 antibody [EPR8736] (ab131285)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8736] to BIG-2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Overview

  • Product name

    Anti-BIG-2 antibody [EPR8736]
    See all BIG-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8736] to BIG-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human BIG-2 aa 800-900.
    Database link: Q8IWV2

  • Positive control

    • Fetal brain lysate, Human cerebellum lysate. Human kidney tissue and Human brain tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8736
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis

Associated products

  • Alternative Versions

    • Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab131285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.
    • Tissue specificity

      Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex.
    • Involvement in disease

      A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. Contactin family.
      Contains 4 fibronectin type-III domains.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Cell membrane. Secreted.
    • Target information above from: UniProt accession Q8IWV2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 152330 Human
      • Entrez Gene: 269784 Mouse
      • Entrez Gene: 116658 Rat
      • Omim: 607280 Human
      • SwissProt: Q8IWV2 Human
      • SwissProt: Q69Z26 Mouse
      • SwissProt: Q62845 Rat
      • Unigene: 298705 Human
      • Unigene: 626418 Human
      • Unigene: 321683 Mouse
      • Unigene: 10117 Rat
      see all
    • Alternative names

      • AXCAM antibody
      • Axonal associated cell adhesion molecule antibody
      • BIG 2 antibody
      • BIG-2 antibody
      • Brain derived immunoglobulin superfamily protein 2 antibody
      • Brain-derived immunoglobulin superfamily protein 2 antibody
      • Cntn4 antibody
      • CNTN4_HUMAN antibody
      • CNTN4A antibody
      • contactin 4 antibody
      • Contactin-4 antibody
      • Neural cell adhesion protein BIG 2 antibody
      • SCA16 antibody
      see all

    Images

    • Western blot - Anti-BIG-2 antibody [EPR8736] (ab131285)
      Western blot - Anti-BIG-2 antibody [EPR8736] (ab131285)
      All lanes : Anti-BIG-2 antibody [EPR8736] (ab131285) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 113 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelled with ab131285 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] (ab131285)

      Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab131285 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-BIG-2 antibody [EPR8736] (ab131285)
      Anti-BIG-2 antibody [EPR8736] (ab131285)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab131285? Please let us know so that we can cite the reference in this datasheet.

    ab131285 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab131285.
    Please use the links above to contact us or submit feedback about this product.

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    For licensing inquiries, please contact partnerships@abcam.com

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