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RecombinantRabMAb

Recombinant Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)

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  • SDS
  • Certificate of Compliance
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Western blot - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
  • Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8736] to BIG-2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-BIG-2 antibody [EPR8736] - BSA and Azide free
    See all BIG-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8736] to BIG-2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248406 is the carrier-free version of ab131285. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab248406 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8736
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis

Associated products

  • Alternative Versions

    • Anti-BIG-2 antibody [EPR8736] (ab131285)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Contactins mediate cell surface interactions during nervous system development. Has some neurite outgrowth-promoting activity. May be involved in synaptogenesis.
    • Tissue specificity

      Mainly expressed in brain. Highly expressed in cerebellum and weakly expressed in corpus callosum, caudate nucleus, amygdala and spinal cord. Also expressed in testis, pancreas, thyroid, uterus, small intestine and kidney. Not expressed in skeletal muscle. Isoform 2 is weakly expressed in cerebral cortex.
    • Involvement in disease

      A chromosomal aberration involving CNTN4 has been found in a boy with characteristic physical features of 3p deletion syndrome (3PDS). Translocation t(3;10)(p26;q26). 3PDS is a rare contiguous gene disorder involving the loss of the telomeric portion of the short arm of chromosome 3 and characterized by developmental delay, growth retardation, and dysmorphic features.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. Contactin family.
      Contains 4 fibronectin type-III domains.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Cell membrane. Secreted.
    • Target information above from: UniProt accession Q8IWV2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 152330 Human
      • Entrez Gene: 269784 Mouse
      • Entrez Gene: 116658 Rat
      • Omim: 607280 Human
      • SwissProt: Q8IWV2 Human
      • SwissProt: Q69Z26 Mouse
      • SwissProt: Q62845 Rat
      • Unigene: 298705 Human
      • Unigene: 626418 Human
      • Unigene: 321683 Mouse
      • Unigene: 10117 Rat
      see all
    • Alternative names

      • AXCAM antibody
      • Axonal associated cell adhesion molecule antibody
      • BIG 2 antibody
      • BIG-2 antibody
      • Brain derived immunoglobulin superfamily protein 2 antibody
      • Brain-derived immunoglobulin superfamily protein 2 antibody
      • Cntn4 antibody
      • CNTN4_HUMAN antibody
      • CNTN4A antibody
      • contactin 4 antibody
      • Contactin-4 antibody
      • Neural cell adhesion protein BIG 2 antibody
      • SCA16 antibody
      see all

    Images

    • Western blot - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      Western blot - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      All lanes : Anti-BIG-2 antibody [EPR8736] (ab131285) at 1/1000 dilution

      Lane 1 : Fetal brain tissue lysate
      Lane 2 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 113 kDa



      This data was developed using ab131285, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      This data was developed using ab131285, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelled with ab131285 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      This data was developed using ab131285, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labelled with ab131285 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)
      Anti-BIG-2 antibody [EPR8736] - BSA and Azide free (ab248406)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab248406? Please let us know so that we can cite the reference in this datasheet.

    ab248406 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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