Product nameAnti-BIG1 antibody
See all BIG1 primary antibodies
DescriptionRabbit polyclonal to BIG1
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to a region between residue 1800 and the C-terminus (residue 1849) of human BIG1 (according to NP_006412.2)
- Whole cell lysate from HeLa, 293T or NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 208 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceBIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
Cellular localizationCytoplasmic, Golgi Apparatus and Nuclear
- ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
- ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
- ARF GEP1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BIG1 with ab72061 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-BIG1 antibody (ab72061) at 0.1 µg/ml
Lane 1 : Whole
cell lysate from HeLa cells at 50 µg
Lane 2 : Whole
cell lysate from HeLa cells at 15 µg
Lane 3 : Whole
cell lysate from HeLa cells at 5 µg
Lane 4 : Whole
cell lysate from 293T cells at 50 µg
Lane 5 : Whole
cell lysate from NIH 3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 208 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of Human BIG1 by Immunoprecipitation in 1mg whole cell lysate from HeLa cells, using ab72061 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). Subsequent Western blot detection was performed using ab72061 at 1 µg/ml on 20% of the imunoprecipitate.
ab72061 has not yet been referenced specifically in any publications.