Recombinant
RabMAb

Anti-BIG1 antibody [EPR10046(2)] (ab183747)

Abreviews (1) Submit a Question

Overview

  • Product name
    Anti-BIG1 antibody [EPR10046(2)]
    See all BIG1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10046(2)] to BIG1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BIG1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y6D6

  • Positive control
    • Jurkat, 293T and A431 cell lysates; Human kidney tissue; 293T and A431 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100.

Target

  • Relevance
    BIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
  • Cellular localization
    Cytoplasmic, Golgi Apparatus and Nuclear
  • Database links
    see all
  • Alternative names
    • ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
    • ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
    • ARF GEP1 antibody
    • ARFGEF1 antibody
    • ARFGEP1 antibody
    • Brefeldin A inhibited GEP 1 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 1 antibody
    • P200 antibody
    • p200 ARF guanine nucleotide exchange factor antibody
    see all

Images

  • Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    All lanes : Anti-BIG1 antibody [EPR10046(2)] (ab183747) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 209 kDa
    Observed band size: 250 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BIG1 with ab183747 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

References

ab183747 has not yet been referenced specifically in any publications.

Publishing research using ab183747? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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Immunoprecipitation abreview for Anti-BIG1 antibody [EPR10046(2)]

 Good
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa Cells)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A
Specification
HeLa Cells
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Username

Mrs. Andrea Geelhaar

Verified customer

Submitted Aug 02 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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