Recombinant Anti-BIG1 antibody [EPR10046(2)] (ab183747)
![Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.com/ps/products/183/ab183747/Images/ab183747-215830-anti-big1-antibody-epr100462-western-blot.jpg "Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10046(2)] to BIG1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BIG1 antibody [EPR10046(2)]
See all BIG1 primary antibodies -
Description
Rabbit monoclonal [EPR10046(2)] to BIG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, 293T and A431 cell lysates; Human kidney tissue; 293T and A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10046(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| Notes |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
Target
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Relevance
BIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes. -
Cellular localization
Cytoplasmic, Golgi Apparatus and Nuclear -
Database links
- Entrez Gene: 10565 Human
- Entrez Gene: 211673 Mouse
- Entrez Gene: 312915 Rat
- Omim: 604141 Human
- SwissProt: Q9Y6D6 Human
- SwissProt: G3X9K3 Mouse
- SwissProt: D4A631 Rat
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Alternative names
- ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
- ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
- ARF GEP1 antibody
see all
Images
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Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)All lanes : Anti-BIG1 antibody [EPR10046(2)] (ab183747) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 209 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.com/ps/products/183/ab183747/Images/ab183747-215829-anti-big1-antibody-epr100462-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BIG1 with ab183747 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.com/ps/products/183/ab183747/Images/ab183747-215828-anti-big1-antibody-epr100462-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.com/ps/products/183/ab183747/Images/ab183747-215827-anti-big1-antibody-epr100462-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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![Anti-BIG1 antibody [EPR10046(2)] (ab183747)](https://www.abcam.com/ps/products/183/ab183747/Images/ab183747-5-benefits-of-recombinant-antibodies.png)
Anti-BIG1 antibody [EPR10046(2)] (ab183747)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab183747 has been referenced in 1 publication.
- Han J et al. miR-215 suppresses papillary thyroid cancer proliferation, migration, and invasion through the AKT/GSK-3ß/Snail signaling by targeting ARFGEF1. Cell Death Dis 10:195 (2019). PubMed: 30814512
