RecombinantRabMAb

Recombinant Anti-BIG1 antibody [EPR10046(2)] (ab183747)

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Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
  • Anti-BIG1 antibody [EPR10046(2)] (ab183747)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10046(2)] to BIG1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BIG1 antibody [EPR10046(2)]
    See all BIG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10046(2)] to BIG1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BIG1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y6D6

  • Positive control

    • Jurkat, 293T and A431 cell lysates; Human kidney tissue; 293T and A431 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab183747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Relevance

    BIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.

  • Cellular localization

    Cytoplasmic, Golgi Apparatus and Nuclear

  • Database links

    see all

  • Alternative names

    • ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
    • ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
    • ARF GEP1 antibody
    • ARFGEF1 antibody
    • ARFGEP1 antibody
    • Brefeldin A inhibited GEP 1 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 1 antibody
    • P200 antibody
    • p200 ARF guanine nucleotide exchange factor antibody
    see all

Images

  • Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    All lanes : Anti-BIG1 antibody [EPR10046(2)] (ab183747) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 209 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BIG1 with ab183747 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Anti-BIG1 antibody [EPR10046(2)] (ab183747)

References (1)

Publishing research using ab183747? Please let us know so that we can cite the reference in this datasheet.

ab183747 has been referenced in 1 publication.

  • Han J  et al. miR-215 suppresses papillary thyroid cancer proliferation, migration, and invasion through the AKT/GSK-3ß/Snail signaling by targeting ARFGEF1. Cell Death Dis 10:195 (2019). PubMed: 30814512

Customer reviews and Q&As

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Immunoprecipitation abreview for Anti-BIG1 antibody [EPR10046(2)]

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa Cells)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A
Specification
HeLa Cells
Read More

Mrs. Andrea Geelhaar

Verified customer

Submitted Aug 02 2017

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