Recombinant
RabMAb

Recombinant Anti-BIG1 antibody [EPR10046(2)] (ab183747)

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Overview

  • Product name

    Anti-BIG1 antibody [EPR10046(2)]
    See all BIG1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10046(2)] to BIG1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human BIG1 aa 1-100. The exact sequence is proprietary.
    Database link: Q9Y6D6

  • Positive control

    • Jurkat, 293T and A431 cell lysates; Human kidney tissue; 293T and A431 cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 250 kDa (predicted molecular weight: 209 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.

Target

  • Relevance

    BIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.

  • Cellular localization

    Cytoplasmic, Golgi Apparatus and Nuclear

  • Database links

    see all

  • Alternative names

    • ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
    • ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
    • ARF GEP1 antibody
    • ARFGEF1 antibody
    • ARFGEP1 antibody
    • Brefeldin A inhibited GEP 1 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 1 antibody
    • P200 antibody
    • p200 ARF guanine nucleotide exchange factor antibody
    see all

Images

  • Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Western blot - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    All lanes : Anti-BIG1 antibody [EPR10046(2)] (ab183747) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 209 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BIG1 with ab183747 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)
    Immunocytochemistry/ Immunofluorescence - Anti-BIG1 antibody [EPR10046(2)] (ab183747)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling BIG1 with ab183747 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

References

This product has been referenced in:

  • Han J  et al. miR-215 suppresses papillary thyroid cancer proliferation, migration, and invasion through the AKT/GSK-3ß/Snail signaling by targeting ARFGEF1. Cell Death Dis 10:195 (2019). Read more (PubMed: 30814512) »
See 1 Publication for this product

Publishing research using ab183747? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunoprecipitation abreview for Anti-BIG1 antibody [EPR10046(2)]

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa Cells)
Total protein in input
1000 µg
Immuno-precipitation step
Protein A
Specification
HeLa Cells
Read More

Mrs. Andrea Geelhaar

Verified customer

Submitted Aug 02 2017

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