Key features and details
- Rabbit polyclonal to Biglycan
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Biglycan antibody
See all Biglycan primary antibodies
DescriptionRabbit polyclonal to Biglycan
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog
Recombinant fragment within Human Biglycan (internal sequence). The exact sequence is proprietary.
Database link: P21810
- WB: HEK-293T whole cell lysate. IHC-P: Human colon epithelium tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionMay be involved in collagen fiber assembly.
Tissue specificityFound in several connective tissues, especially in articular cartilages.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
Paraffin-embedded human colon epithelium tissue stained for Biglycan using ab226991 at 1/250 dilution in immunohistochemical analysis.
Anti-Biglycan antibody (ab226991) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 42 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226991 has not yet been referenced specifically in any publications.