Product nameAnti-Biglycan antibody
See all Biglycan primary antibodies
DescriptionRabbit polyclonal to Biglycan
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
- This antibody gave a positive signal in the following Lysates: Human Skin Tissue HepG2 Whole Cell Jurkat Whole Cell THP1 Whole Cell Rat Spleen Tissue Rat Thymus Tissue Mouse Kidney Tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab49701 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionMay be involved in collagen fiber assembly.
Tissue specificityFound in several connective tissues, especially in articular cartilages.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
All lanes : Anti-Biglycan antibody (ab49701) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 :
THP1 whole cell lysate (ab7913)
Lane 5 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)
Lane 6 : Thymus (Rat) Tissue Lysate
Lane 7 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Additional bands at: 26 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
IHC image of Biglycan staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49701, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab49701 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49701, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Erndt-Marino JD et al. In vitro evaluation of a basic fibroblast growth factor-containing hydrogel toward vocal fold lamina propria scar treatment. J Biomed Mater Res B Appl Biomater 106:1258-1267 (2018). Read more (PubMed: 28580765) »
- Andrlová H et al. Biglycan expression in the melanoma microenvironment promotes invasiveness via increased tissue stiffness inducing integrin-ß1 expression. Oncotarget 8:42901-42916 (2017). Read more (PubMed: 28476030) »