• Product name

  • Description

    Rabbit polyclonal to Biglycan
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Biglycan.

    Read Abcam's proprietary immunogen policy (Peptide available as ab69790.)

  • Positive control

    • This antibody gave a positive signal in the following Lysates: Human Skin Tissue HepG2 Whole Cell Jurkat Whole Cell THP1 Whole Cell Rat Spleen Tissue Rat Thymus Tissue Mouse Kidney Tissue This antibody gave a positive result in IHC in the following FFPE tissue: Human placenta.



Our Abpromise guarantee covers the use of ab49701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.


  • Function

    May be involved in collagen fiber assembly.
  • Tissue specificity

    Found in several connective tissues, especially in articular cartilages.
  • Sequence similarities

    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • Post-translational

    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SEMDX antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all


  • All lanes : Anti-Biglycan antibody (ab49701) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : THP1 whole cell lysate (ab7913)
    Lane 5 : Spleen (Rat) Tissue Lysate - normal tissue (ab29393)
    Lane 6 : Thymus (Rat) Tissue Lysate
    Lane 7 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 26 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes
  • IHC image of Biglycan staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49701, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab49701 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49701, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Erndt-Marino JD  et al. In vitro evaluation of a basic fibroblast growth factor-containing hydrogel toward vocal fold lamina propria scar treatment. J Biomed Mater Res B Appl Biomater 106:1258-1267 (2018). Read more (PubMed: 28580765) »
  • Andrlová H  et al. Biglycan expression in the melanoma microenvironment promotes invasiveness via increased tissue stiffness inducing integrin-ß1 expression. Oncotarget 8:42901-42916 (2017). Read more (PubMed: 28476030) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Pig Tissue sections (nasal septum)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 0,3 % Collagenase & 0,5 U/mL Chondroitinase ABC
nasal septum
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Katharina Tluczynski

Verified customer

Submitted Apr 25 2016

Western blot
Pig Cell lysate - whole cell (Porcine Vocal Fold Fibroblasts (VFF))
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
1 µg
Porcine Vocal Fold Fibroblasts (VFF)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 05 2015

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