Overview

  • Product name

  • Description

    Goat polyclonal to Biglycan
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Sheep, Horse, Dog, Xenopus laevis, Pufferfish, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human Biglycan aa 357-368 (C terminal).
    Sequence:

    C-DRLAIQFGNYKK


    Database link: P21810
    (Peptide available as ab119271)

  • Positive control

    • Rat Skin lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use at an assay dependent concentration.

Target

Images

  • Immunocytochemistry/immunofluorescent analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized HepG2 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).

    Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).

  • Immunocytochemistry/immunofluorescent analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized NIH3T3 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).

    Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).

  • Anti-Biglycan antibody (ab58562) at 0.1 µg/ml + Rat Skin lysate (35µg protein in RIPA buffer).

    Predicted band size: 42 kDa
    Observed band size: 42 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab58562 staining Biglycan in mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with acetone and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS with 1% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated donkey anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Yokoi M  et al. Effect of attenuation of fibroblast growth factor receptor 2b signaling on odontoblast differentiation and dentin formation. In Vitro Cell Dev Biol Anim 55:211-219 (2019). Read more (PubMed: 30756235) »
  • Zadrozny LM  et al. Study of the development of the mouse thoracic aorta three-dimensional macromolecular structure using two-photon microscopy. J Histochem Cytochem 63:8-21 (2015). Read more (PubMed: 25362141) »
See all 15 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pulmonary valve leaflet)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Envision Flex Target Retrieval Solution High pH
Permeabilization
No
Specification
Pulmonary valve leaflet
Blocking step
Rabbit Serum (Normal) plus Rabbit F(ab) anti-Sheep IgG (ab136726) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100µg/mL · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Oct 07 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Cartilage)
Specification
Cartilage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 12 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Aorta)
Specification
Aorta
Treatment
PNGaseF
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jan 17 2014

Answer

Thank you for your email. We do provide the corresponding peptide for ab58562 Anti-Biglycan antibody. The product is ab119271 Biglycan peptide: https://www.abcam.com/ab119271 Unfortunately we do not currently stock the peptide for ab108930. Please do not hesitate to contact me should you have further questions.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: low pH 7.0 TRS and high pH 9.0 TRS-9 from Dako 120C for 30sec
Permeabilization
No
Blocking step
DEEB (dual endogenous enzyme block for autostain) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. Leann Mikesh

Verified customer

Submitted Jul 06 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 18 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up