Anti-Biglycan antibody (ab58562)
Key features and details
- Goat polyclonal to Biglycan
- Suitable for: ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Biglycan antibody
See all Biglycan primary antibodies -
Description
Goat polyclonal to Biglycan -
Host species
Goat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Horse, Dog, Xenopus laevis, Pufferfish, Zebrafish -
Immunogen
Synthetic peptide within Human Biglycan aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P21810 -
Positive control
- ICC: Human HepG2 and Mouse NIH/3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 µg/ml.
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Notes |
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ICC
Use a concentration of 10 µg/ml. |
Target
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Function
May be involved in collagen fiber assembly. -
Tissue specificity
Found in several connective tissues, especially in articular cartilages. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
Post-translational
modificationsThe two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 403905 Dog
- Entrez Gene: 100033879 Horse
- Entrez Gene: 633 Human
- Entrez Gene: 12111 Mouse
- Entrez Gene: 25181 Rat
- Entrez Gene: 443011 Sheep
- Entrez Gene: 399351 Xenopus laevis
- Omim: 301870 Human
see all -
Alternative names
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
see all
Images
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Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized HepG2 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).
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Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized NIH3T3 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).
Protocols
Datasheets and documents
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Datasheet download
References (20)
ab58562 has been referenced in 20 publications.
- Yin Y et al. Bmi1 regulate tooth and mandible development by inhibiting p16 signal pathway. J Cell Mol Med 25:4195-4203 (2021). PubMed: 33745198
- Zhou C et al. Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease. Aging (Albany NY) 11:7257-7273 (2019). PubMed: 31509519
- Yokoi M et al. Effect of attenuation of fibroblast growth factor receptor 2b signaling on odontoblast differentiation and dentin formation. In Vitro Cell Dev Biol Anim 55:211-219 (2019). PubMed: 30756235
- Murphy S et al. Proteomic profiling of the dystrophin complex and membrane fraction from dystrophic mdx muscle reveals decreases in the cytolinker desmoglein and increases in the extracellular matrix stabilizers biglycan and fibronectin. J Muscle Res Cell Motil 38:251-268 (2017). PubMed: 28803268
- Hsieh CF et al. Periodontal ligament cells as alternative source for cell-based therapy of tendon injuries: in vivo study of full-size Achilles tendon defect in a rat model. Eur Cell Mater 32:228-240 (2016). PubMed: 27763655