Overview

  • Product name

  • Description

    Rabbit polyclonal to Biglycan
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SEAKLTGIPK DLPETLNELH LDHNKIQAIE LEDLLRYSKL YRLGLGHNQI) of Human Biglycan (NP_001702).

  • Positive control

    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-Biglycan antibody (ab94460) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 42 kDa



    Gel concentration: 12%

References

ab94460 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Bos taurus Purified protein (Conditioned medium from vascular endothelial cells)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
550000 cells
Treatment
Chondroitinase ABC (1.5mU/µL) for 3 hrs
Specification
Conditioned medium from vascular endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 03 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Cardiomyocyte)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
Cardiomyocyte
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jan 12 2018

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Tissue lysate - whole (Aorta)
Specification
Aorta
Treatment
Non deglycosylated - Deglycosylated (PNGaseF)
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 03 2014

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