Product nameAnti-Biglycan antibody [EPR2959]
See all Biglycan primary antibodies
DescriptionRabbit monoclonal [EPR2959] to Biglycan
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Biglycan (C terminal). The exact sequence is proprietary.
- Rat cartilage, C2C12, Human skeletal muscle and Mouse muscle lysates; L6 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay be involved in collagen fiber assembly.
Tissue specificityFound in several connective tissues, especially in articular cartilages.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
modificationsThe two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
Flow cytometric analysis of permeabilized L6 cells using ab109369 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
All lanes : Anti-Biglycan antibody [EPR2959] (ab109369) at 1/1000 dilution
Lane 1 : Rat cartilage lysate
Lane 2 : L6 lysate
Lane 3 : C2C12 lysate
Lane 4 : Human skeletal muscle lysate
Lane 5 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa
This product has been referenced in:
- Liu S et al. Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study. Oncotarget 7:75468-75481 (2016). WB ; Human . Read more (PubMed: 27690342) »
- Tan HT et al. Unravelling the proteome of degenerative human mitral valves. Proteomics 15:2934-44 (2015). WB ; Human . Read more (PubMed: 25914152) »