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Rabbit monoclonal [EPR2959] to Biglycan
, Flow Cytmore detailsUnsuitable for:
ICC or IHC-P
Mouse, Rat, Human
Synthetic peptide within Human Biglycan (C terminal). The exact sequence is proprietary.
- Rat cartilage, C2C12, Human skeletal muscle and Mouse muscle lysates; L6 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
||1/10 - 1/100.
||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC or IHC-P.
May be involved in collagen fiber assembly.
Found in several connective tissues, especially in articular cartilages.
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats.
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
Secreted > extracellular space > extracellular matrix.
Information by UniProt
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
Flow Cytometry - Anti-Biglycan antibody [EPR2959] (ab109369)
Flow cytometric analysis of permeabilized L6 cells using ab109369 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Western blot - Anti-Biglycan antibody [EPR2959] (ab109369)
All lanes : Anti-Biglycan antibody [EPR2959] (ab109369) at 1/1000 dilution
Lane 1 : Rat cartilage lysate
Lane 2 : L6 lysate
Lane 3 : C2C12 lysate
Lane 4 : Human skeletal muscle lysate
Lane 5 : Mouse muscle lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"