Overview

  • Product name

  • Description

    Rabbit polyclonal to BIII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit
  • Immunogen

    Other Immunogen Type corresponding to Human BIII aa 2013-2179.
    Database link: NP_000709

  • Positive control

    • A549 whole cell lysate and HCT116 cell lysate IHC-P: FFPE human cerebral cortex tissue sections.
  • General notes

    Previously labelled as CACNA1B (N type).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB 1/1000. Predicted molecular weight: 263 kDa.

Target

  • Relevance

    Voltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.
  • Cellular localization

    Membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • BIII antibody
    • Brain calcium channel III antibody
    • CACH5 antibody
    • CACNA1B (N type) antibody
    • CACNL1A5 antibody
    • CACNN antibody
    • Calcium channel alpha12.2 subunit antibody
    • Calcium channel L type alpha 1 polypeptide isoform 5 antibody
    • Calcium channel L type, alpha 1 polypeptide antibody
    • Calcium channel N type antibody
    • Calcium channel voltage dependent alpha 1B subunit N type antibody
    • Calcium channel voltage dependent L type alpha 1B subunit antibody
    • Calcium channel voltage dependent N type alpha 1B subunit antibody
    • Cav2.2 antibody
    • Cav2.2 voltage gated Ca2+ channel antibody
    • Voltage dependent N type calcium channel subunit alpha 1B antibody
    • Voltage gated calcium channel alpha subunit Cav2.2 antibody
    see all

Images

  • IHC image of BIII staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-BIII antibody (ab97284) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 263 kDa



    5% SDS PAGE

References

This product has been referenced in:

  • Li Y  et al. Validation and bioinformatic analysis of propofol-induced differentially expressed microRNAs in primary cultured neural stem cells. Gene 664:90-100 (2018). WB . Read more (PubMed: 29679758) »
  • Lin JJ  et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). WB . Read more (PubMed: 28656058) »
See all 2 Publications for this product

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