Product nameAnti-BIII antibody
See all BIII primary antibodies
DescriptionRabbit polyclonal to BIII
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Rabbit
Other Immunogen Type corresponding to Human BIII aa 2013-2179.
Database link: NP_000709
- A549 whole cell lysate and HCT116 cell lysate IHC-P: FFPE human cerebral cortex tissue sections.
Previously labelled as CACNA1B (N type).
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||1/1000. Predicted molecular weight: 263 kDa.|
RelevanceVoltage-sensitive calcium channels (VSCCs) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death.
Cellular localizationMembrane; Multi-pass membrane protein.
- BIII antibody
- Brain calcium channel III antibody
- CACH5 antibody
IHC image of BIII staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97284, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-BIII antibody (ab97284) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 263 kDa
5% SDS PAGE
This product has been referenced in:
- Li Y et al. Validation and bioinformatic analysis of propofol-induced differentially expressed microRNAs in primary cultured neural stem cells. Gene 664:90-100 (2018). WB . Read more (PubMed: 29679758) »
- Lin JJ et al. Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice. Theranostics 7:2015-2032 (2017). WB . Read more (PubMed: 28656058) »