Recombinant Anti-BIII antibody [EPR25384-127] (ab288155)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25384-127] to BIII
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-BIII antibody [EPR25384-127]
See all BIII primary antibodies -
Description
Rabbit monoclonal [EPR25384-127] to BIII -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain, Mouse hippocampus, Rat brain, Rat hippocampus and Human cerebellum lysates. IHC-P: Mouse cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25384-127 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Unsuitable with Rat and Human. |
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WB |
1/1000. Detects a band of approximately 75-150 kDa (predicted molecular weight: 262 kDa).
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Unsuitable with Rat and Human. |
WB
1/1000. Detects a band of approximately 75-150 kDa (predicted molecular weight: 262 kDa). |
Target
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Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons. -
Tissue specificity
Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta. -
Involvement in disease
Dystonia 23 -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1B subfamily.
Contains 1 EF-hand domain. -
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 774 Human
- Entrez Gene: 12287 Mouse
- Entrez Gene: 257648 Rat
- Omim: 601012 Human
- SwissProt: Q00975 Human
- SwissProt: O55017 Mouse
- SwissProt: Q02294 Rat
- Unigene: 495522 Human
see all -
Alternative names
- alpha-1 polypeptide isoform 5 antibody
- BIII antibody
- Brain calcium channel III antibody
see all
Images
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All lanes : Anti-BIII antibody [EPR25384-127] (ab288155) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate at 20 µg
Lane 2 : Mouse hippocampus tissue lysate at 20 µg
Lane 3 : Mouse liver tissue lysate at 40 µg
Lane 4 : Rat brain tissue lysate at 20 µg
Lane 5 : Rat hippocampus tissue lysate at 20 µg
Lane 6 : Rat liver tissue lysate at 40 µg
Lane 7 : Human cerebellum tissue lysate at 20 µg
Lane 8 : Human liver tissue lysate at 40 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 262 kDa
Observed band size: 262,75-150 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Bis-Tris gel was used for this blot.
Samples are non-boiled as boiling may cause protein aggregates.
The lower bands (75-150kda) may represent different isoforms or proteolytic fragments according to one new study on KO mice (PMID:34353899).
Negative control: liver
Exposure time: Lanes 1-6: 37 seconds Lanes 7-8: 81 seconds
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Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling BIII with ab288155 at 1/100 (4.84 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in mouse cerebrum.The section was incubated with ab288155 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling BIII with ab288155 at 1/100 (4.84 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse liver. The section was incubated with ab288155 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab288155 has not yet been referenced specifically in any publications.