Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-Bik antibody (ab52182)Image courtesy of an anonymous Abreview.
ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.