Product nameAnti-Bik antibody
See all Bik primary antibodies
DescriptionRabbit polyclonal to Bik
SpecificityDetects endogenous levels of total BIK protein.
Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic non-phosphopeptide (human) around the phosphorylation site of threonine 33 (G-M-T-D-S).
- Extracts from A549 cells treated with DMSO, human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS, 0.87% Sodium chloride
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 18 kDa.|
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
FunctionAccelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localizationEndomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
- Information by UniProt
- Apoptosis inducer NBK antibody
- BBC1 antibody
- Bcl-2-interacting killer antibody
All lanes : Anti-Bik antibody (ab52182) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with DMSO (0.1%, 10mins)
Lane 2 : extracts from A549 cells, treated
with DMSO (0.1%, 10mins) with blocking peptide
Predicted band size: 18 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Ab52182 at 1/50 dilution staining Human breast carcinoma tissue, with and without blocking peptide; paraffin embedded.
ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.
This product has been referenced in:
- Wang X et al. Synergistic control of sex hormones by 17ß-HSD type 7: a novel target for estrogen-dependent breast cancer. J Mol Cell Biol 7:568-79 (2015). Read more (PubMed: 25966904) »
- Wu J et al. Silencing of Kv1.5 Gene Inhibits Proliferation and Induces Apoptosis of Osteosarcoma Cells. Int J Mol Sci 16:26914-26 (2015). WB ; Human . Read more (PubMed: 26569226) »