Overview

  • Product name

    Anti-Bik antibody - Aminoterminal end
    See all Bik primary antibodies
  • Description

    Rabbit polyclonal to Bik - Aminoterminal end
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with Bik
  • Tested applications

    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A 15 amino acid synthetic peptide from near the amino terminus of human Bik

  • Positive control

    • Jurkat whole cell lysate (ab7899) and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
ICC Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function

    Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
  • Domain

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization

    Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis inducer NBK antibody
    • BBC1 antibody
    • Bcl-2-interacting killer antibody
    • BCL2 interacting killer antibody
    • bhikhari antibody
    • BIK antibody
    • Bik-like killer protein antibody
    • BIK_HUMAN antibody
    • BIP 1 antibody
    • BiP1 antibody
    • BP 4 antibody
    • BP4 antibody
    • cb 60 antibody
    • NBK antibody
    see all

Images

  • Lane 1 : Anti-Bik antibody - Aminoterminal end (ab62494) at 1 µg/ml
    Lane 2 : Anti-Bik antibody - Aminoterminal end (ab62494) at 2 µg/ml

    All lanes : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 18 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry of Bik in Jurkat cells with ab62494 antibody at 1 µg/ml.
  • Immunofluorescence of Bik in Jurkat cells using ab62494 at 10 ug/ml.

References

ab62494 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab62494.
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