Key features and details
- Rabbit polyclonal to Bik - Aminoterminal end
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Bik antibody - Aminoterminal end
See all Bik primary antibodies
DescriptionRabbit polyclonal to Bik - Aminoterminal end
SpecificityThis antibody reacts with Bik
Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
A 15 amino acid synthetic peptide from near the amino terminus of human Bik
- Jurkat whole cell lysate (ab7899) and Jurkat cells
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).|
|ICC||Use a concentration of 1 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionAccelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localizationEndomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
- Information by UniProt
- Apoptosis inducer NBK antibody
- BBC1 antibody
- Bcl-2-interacting killer antibody
Lane 1 : Anti-Bik antibody - Aminoterminal end (ab62494) at 1 µg/ml
Lane 2 : Anti-Bik antibody - Aminoterminal end (ab62494) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Immunocytochemistry of Bik in Jurkat cells with ab62494 antibody at 1 µg/ml.
Immunofluorescence of Bik in Jurkat cells using ab62494 at 10 ug/ml.
ab62494 has not yet been referenced specifically in any publications.