Overview

  • Product name

    Anti-Bikunin + Trypstatin antibody
  • Description

    Rabbit polyclonal to Bikunin + Trypstatin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-262 of Human Bikunin + Trypstatin (UniProt: P02760).

  • Positive control

    • 293T and A431 whole cell lysates; Human Hepatoma tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab153806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • Tissue specificity

    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • Sequence similarities

    In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • Post-translational
    modifications

    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A1M antibody
    • Alpha 1 microglobulin/bikunin precursor antibody
    • Alpha-1 microglycoprotein antibody
    • AMBP antibody
    • AMBP_HUMAN antibody
    • Bikunin antibody
    • Complex-forming glycoprotein heterogeneous in charge antibody
    • EDC1 antibody
    • Growth inhibiting protein 19 antibody
    • HCP antibody
    • HI-30 antibody
    • HI30 antibody
    • IATIL antibody
    • Inter alpha trypsin inhibitor light chain antibody
    • ITI antibody
    • ITI-LC antibody
    • ITIL antibody
    • ITILC antibody
    • Protein AMBP antibody
    • Protein HC antibody
    • Trypstatin antibody
    • Uristatin antibody
    • Uronic-acid-rich protein antibody
    • UTI antibody
    see all

Images

  • All lanes : Anti-Bikunin + Trypstatin antibody (ab153806) at 1/5000 dilution

    Lane 1 : 293T whole cell
    lysate
    Lane 2 : A431 whole cell
    lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 39 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling Bikunin + Trypstatin with ab153806 at 1/500 dilution.

References

This product has been referenced in:

  • Lauer ME  et al. Modification of hyaluronan by heavy chains of inter-a-inhibitor in idiopathic pulmonary arterial hypertension. J Biol Chem 289:6791-8 (2014). Read more (PubMed: 24403074) »
See 1 Publication for this product

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