Product nameAnti-Bikunin + Trypstatin antibody
DescriptionRabbit polyclonal to Bikunin + Trypstatin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to a region within amino acids 1-262 of Human Bikunin + Trypstatin (UniProt: P02760).
- 293T and A431 whole cell lysates; Human Hepatoma tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153806 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 39 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionInter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Trypstatin is a trypsin inhibitor.
Tissue specificityExpressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
Sequence similaritiesIn the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
Contains 2 BPTI/Kunitz inhibitor domains.
modificationsThe precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
- Information by UniProt
- A1M antibody
- Alpha 1 microglobulin/bikunin precursor antibody
- Alpha-1 microglycoprotein antibody
All lanes : Anti-Bikunin + Trypstatin antibody (ab153806) at 1/5000 dilution
Lane 1 : 293T whole cell
Lane 2 : A431 whole cell
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
10% SDS PAGE
Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling Bikunin + Trypstatin with ab153806 at 1/500 dilution.
ab153806 has been referenced in 1 publication.
- Lauer ME et al. Modification of hyaluronan by heavy chains of inter-a-inhibitor in idiopathic pulmonary arterial hypertension. J Biol Chem 289:6791-8 (2014). PubMed: 24403074