Product nameAnti-Bile Acid Receptor NR1H4 antibody [3188.8.131.52] - N-terminal
See all Bile Acid Receptor NR1H4 primary antibodies
DescriptionMouse monoclonal [3184.108.40.206] to Bile Acid Receptor NR1H4 - N-terminal
Tested applicationsSuitable for: ICC/IF, Flow Cyt, ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human liver tissue; Bile Acid Receptor NR1H4-transfected Alexander cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
Our Abpromise guarantee covers the use of ab187735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 8 µg/ml. Predicted molecular weight: 56 kDa.|
|IHC-P||Use a concentration of 20 µg/ml.|
FunctionLigand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- BAR antibody
- Bile acid receptor antibody
- Farnesoid X activated receptor antibody
All lanes : Anti-Bile Acid Receptor NR1H4 antibody [3220.127.116.11] - N-terminal (ab187735) at 8 µg/ml
Lane 1 : Alexander cell lysate
Lane 2 : Alexander cell lysate transfected with Bile Acid Receptor NR1H4
All lanes : HRP-labeled goat anti-mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Bile Acid Receptor NR1H4 with ab187735 at 20 µg/ml.
Immunofluorescent analysis of Bile Acid Receptor NR1H4-transfected Alexander cells labeling Bile Acid Receptor NR1H4 with ab187735 at 10 µg/ml.
Flow Cytometry of Alexander cells.
Gray line: control cells
Black line: cells transfected with FXRa
Transfection efficiency: 60%
ab187735 has been referenced in 1 publication.
- Xie G et al. Dysregulated bile acid signaling contributes to the neurological impairment in murine models of acute and chronic liver failure. EBioMedicine 37:294-306 (2018). PubMed: 30344125