Key features and details
- Rabbit polyclonal to Bile Acid Receptor NR1H4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bile Acid Receptor NR1H4 antibody
See all Bile Acid Receptor NR1H4 primary antibodies
DescriptionRabbit polyclonal to Bile Acid Receptor NR1H4
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Hamster, Cow
Synthetic peptide corresponding to Bile Acid Receptor NR1H4 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: HepG2 Whole Cell; Human Kidney Tissue; Human Small Intestine.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab85606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).|
FunctionLigand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- BAR antibody
- Bile acid receptor antibody
- Farnesoid X activated receptor antibody
All lanes : Anti-Bile Acid Receptor NR1H4 antibody (ab85606) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
ICC/IF image of ab85606 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85606, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa and HepG2 cells at 5µg/ml.
ab85606 has not yet been referenced specifically in any publications.