Key features and details
- Rabbit polyclonal to Bile Acid Receptor NR1H4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bile Acid Receptor NR1H4 antibody
See all Bile Acid Receptor NR1H4 primary antibodies
DescriptionRabbit polyclonal to Bile Acid Receptor NR1H4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Three synthetic peptides (human) conjugated to KLH
- An induced culture of E. coli
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Orphan Nuclear Receptors
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab86025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLigand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- BAR antibody
- Bile acid receptor antibody
- Farnesoid X activated receptor antibody
All lanes : Anti-Bile Acid Receptor NR1H4 antibody (ab86025) at 1/1000 dilution
Lane 1 : An un-induced culture of E. coli
Lane 2 : An induced culture of E. coli
Lysates/proteins at 1/5000 dilution per lane.
All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted?
Exposure time: 60 minutes
Gel concentration 10%
ab86025 has not yet been referenced specifically in any publications.