Product nameAnti-Bile salt-activated lipase antibody
See all Bile salt-activated lipase primary antibodies
DescriptionRabbit polyclonal to Bile salt-activated lipase
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Non human primates
Synthetic peptide corresponding to Bile salt-activated lipase aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse Pancreas; Human Pancreas
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab79131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).|
RelevanceDefects in Bile salt activated lipase (CEL) are a cause of maturity onset diabetes of the young type 8 with exocrine dysfunction (MODY8) [MIM:609812], also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY [MIM:606391] is an autosomal dominant form of diabetes mellitus. The pancreas serves both endocrine and exocrine functions. The endocrine cells are found in the islets of Langerhans. They synthesize insulin and other hormones, and are involved in the pathogenesis of diabetes mellitus. The exocrine cells produce bicarbonate and digestive enzymes and are involved in the pathogenesis of pancreatic malabsorption. The localization of the islets within exocrine pancreatic tissue is suggestive of an interdependency and cross talk between these two cell populations in their normal and in their abnormal function.
Cellular localizationCytoplasmic and Nuclear
- BAL antibody
- Bile salt-stimulated lipase antibody
- BSDL antibody
All lanes : Anti-Bile salt-activated lipase antibody (ab79131) at 1 µg/ml
Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
Lane 2 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
We hypothesize that the 74 kDa band represents the mature form of Bile salt-activated lipase, which has had its signal sequenced cleaved off.
IHC image of Bile salt-activated lipase staining in mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79131, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.