Bilirubin Assay Kit (Total and Direct, Colorimetric) (ab235627)

Overview

  • Product name

    Bilirubin Assay Kit (Total and Direct, Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Serum
  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Human
  • Product overview

    Bilirubin (Total and Direct) Colorimetric Assay Kit (ab235627) utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo-salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm).

  • Notes

    Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert’s syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Bilirubin Reagent 1 1 x 2.5ml
    Bilirubin Reagent 2 1 x 1ml
    Total Bilirubin Probe 1 x 10ml
    Direct Bilirubin Probe 1 x 20ml
    Catalyst 1 x 15ml
    Bilirubin Standard (0.2 µg/µl)  2 x 200µl
    DMSO (Anhydrous) 1 x 3.5ml

Images

  • Different volumes of human serum (10 - 50 µL) were assayed following kit protocols. Reported concentrations (in mg/dL): Total Bilirubin: 4; Direct Bilirubin: 1.5. Experimental concentrations (calculated as the average of estimated bilirubin in five different human serum volumes ranging from 10 to 50 µL): Total: 3.8 mg/dL; Direct: 1.75 mg/dL.

Protocols

References

ab235627 has not yet been referenced specifically in any publications.

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