The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
1/25. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Belongs to the Bcl-2 family.
The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
ab15184, at 1:25 dilution, staining Bim in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
Western blot - Anti-Bim antibody (ab15184)Image from Dr JT Lynch et al, Mol Cancer. 2010 Feb 15;9:38, Fig 5.
All lanes : Anti-Bim antibody (ab15184)
Lane 1 : Total cell protein extracts prepared from CEMC7-14 cells, un-treated. Lane 2 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 2 hours. Lane 3 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 6 hours. Lane 4 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 24 hours.
Cells were grown at 37°C and 5% CO2. CEMC7-14 cells were cultured in RPMI medium and all media supplemented with 10% fetal bovine serum and 10 units/ml each of penicillin and streptomycin. DCC treated serum was used in all experiments before dexamethasone treatment.
Li Y et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med13:e1002200 (2016).
Read more (PubMed: 27997540) »
Lynch JT et al. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer9:38 (2010).
Read more (PubMed: 20156337) »