Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.|
|IHC-P||1/25. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionInduces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Tissue specificityIsoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainThe BH3 motif is required for Bcl-2 binding and cytotoxicity.
Cellular localizationMitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
- Information by UniProt
- BCL2 like 11 antibody
- B2L11_HUMAN antibody
- BAM antibody
ab15184, at 1:25 dilution, staining Bim in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
All lanes : Anti-Bim antibody (ab15184)
Lane 1 : Total cell protein extracts prepared from CEMC7-14 cells, un-treated.
Lane 2 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 2 hours.
Lane 3 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 6 hours.
Lane 4 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 24 hours.
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 13 kDa (possible isoform), 16 kDa (possible isoform)
Cells were grown at 37°C and 5% CO2. CEMC7-14 cells were cultured in RPMI medium and all media supplemented with 10% fetal bovine serum and 10 units/ml each of penicillin and streptomycin. DCC treated serum was used in all experiments before dexamethasone treatment.
This product has been referenced in:
- Li Y et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med 13:e1002200 (2016). WB ; Human . Read more (PubMed: 27997540) »
- Lynch JT et al. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer 9:38 (2010). WB ; Human . Read more (PubMed: 20156337) »