Key features and details
- Rabbit polyclonal to Bim
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Bim antibody
See all Bim primary antibodies
DescriptionRabbit polyclonal to Bim
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Bim aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O43521
- HeLa cells. Placenta
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.|
|IHC-P||1/25. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionInduces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Tissue specificityIsoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Sequence similaritiesBelongs to the Bcl-2 family.
DomainThe BH3 motif is required for Bcl-2 binding and cytotoxicity.
Cellular localizationMitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
- Information by UniProt
- BCL2 like 11 antibody
- B2L11_HUMAN antibody
- BAM antibody
ab15184, at 1:25 dilution, staining Bim in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
All lanes : Anti-Bim antibody (ab15184)
Lane 1 : Total cell protein extracts prepared from CEMC7-14 cells, un-treated.
Lane 2 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 2 hours.
Lane 3 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 6 hours.
Lane 4 : Total cell protein extracts prepared from CEMC7-14 cells, treated with dexamethasone (1µM) for 24 hours.
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 13 kDa (possible isoform), 16 kDa (possible isoform)
Cells were grown at 37°C and 5% CO2. CEMC7-14 cells were cultured in RPMI medium and all media supplemented with 10% fetal bovine serum and 10 units/ml each of penicillin and streptomycin. DCC treated serum was used in all experiments before dexamethasone treatment.
ab15184 has been referenced in 5 publications.
- Rachakhom W et al. Dihydrochalcone Derivative Induces Breast Cancer Cell Apoptosis via Intrinsic, Extrinsic, and ER Stress Pathways but Abolishes EGFR/MAPK Pathway. Biomed Res Int 2019:7298539 (2019). PubMed: 31772936
- Cao S et al. The upregulation of miR-101 promotes vascular endothelial cell apoptosis and suppresses cell migration in acute coronary syndrome by targeting CDH5. Int J Clin Exp Pathol 12:3320-3328 (2019). PubMed: 31934175
- Li Y et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med 13:e1002200 (2016). WB ; Human . PubMed: 27997540
- Lynch JT et al. The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer 9:38 (2010). WB ; Human . PubMed: 20156337
- Bouchard C et al. FoxO transcription factors suppress Myc-driven lymphomagenesis via direct activation of Arf. Genes Dev 21:2775-87 (2007). PubMed: 17974917