Overview

  • Product name

  • Description

    Rabbit polyclonal to Bim
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human Bim aa 134-160 conjugated to keyhole limpet haemocyanin.
    Sequence:

    MRQAEPADMRPEIWIAQELRRIGDEFN


    Database link: O43521

  • Positive control

    • WB: K562 and HL-60 cell lysates. IHC-P: Human tonsil tissue. Flow cytometry: WiDr cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab230531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificity

    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localization

    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Images

  • All lanes : Anti-Bim antibody (ab230531) at 1/1000 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 2 : HL-60 (human promyelocytic leukemia cell line) cell lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 22 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bim with ab230531 at 1/50 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of WiDr cells labeling Bim with ab230531 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230531 has not yet been referenced specifically in any publications.

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