Overview

  • Product name
  • Description
    Rabbit polyclonal to Bim
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide corresponding to amino acids 22 to 40 of human origin. The sequence is identical to that of mouse and differs from that of rat by one amino acid.

  • Positive control
    • Human K562 cell lysate

Applications

Our Abpromise guarantee covers the use of ab7888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.Can be blocked with Bim peptide (ab7887).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificity
    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localization
    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Images

  • All lanes : Anti-Bim antibody (ab7888) at 1 µg/ml

    Lane 1 : K562 whole cell lysate
    Lane 2 : A549 whole cell lysate

    Predicted band size: 23 kDa
    Observed band size: 23 kDa

  • ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P

  • ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
See all 10 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab32158.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Would you be able to clarify a few points for me?

When you say that the antibody wasn't working, was the issue that you were not seeing any bands at all? Have you probed the same membranes for antibodies to other targets (i.e. loading control)?

Do you have access to K562 or A549 whole cell lysates to use as a positive control?

Was the primary antibody incubated overnight at 4C?

How was the antibody stored?

I took a look at our stocks and unfortunately we do not have any more of the lot you purchased in September (GR85357-1). We only have lot GR65948-2 in stock now.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. Please let me know how you would like to proceed.

I hope this information is helpful, and I thank you for your cooperation.

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Answer

Thank you for your reply.


I am inclined to believe that the issue is with the samples themselves. It is very unusual for 3 different antibodies to detect bands mainly above 100kDa when the expected MW should be around 25kDa. How long are the samples boiled before loading? In reading your protocol, I could only find 1 minute, which is not sufficient. Boiling 5-10 min is advised to completely denature the proteins.


How do the samples look if you stain the gel with Coomassie? or Ponceau S?

Furthermore, for at least GADD153, expression must be induced in your samples. Did you do this? Also, ab54740 is only validated for human and may not work with your MEFs. Are you running any sort of a loading control to confirm that the procedure is working with other antibodies?

I hope this information is helpful. Please do not hesitate to contact me if you have additional questions.

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Answer

Thank you for your reply.

I was wondering if you would clarify a few additional points for me.


How were the samples prepared? How much is loaded on the gel?


What is the molecular weight of each band in the ladder you provided?


Regarding the WB protocol, each protein may have slightly different requirements, so it is important to consider these when interpreting your results. For example, GADD153 must be induced to be detected by WB with this antibody - how did you treat your cells? Additionally, background is expected with this antibody and a 2hr block with milk is recommended.


Can you confirm if the secondary antibody you have used with these antibodies is made fresh? Your protocol indicated that your lab does reuse secondary antibody, but we do not recommend it. Have you performed no primary controls to be sure your results are due to the primary antibodies?


I look forward to your reply so that I may assist you further. I think some modifications to your protocol will help your results with these antibodies. Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you for contacting Abcam.


I am sorry that you have been experiencing difficulties with these antibodies in WB.


In order to assist you further, I am hoping you can provide some additional details regarding your experiments. Would you please send me a labeled image of your blot, detailed protocol, and sample type and preparation information? Once I have these details, I would be happy to offer some protocol suggestions to improve your results or offer compensation in accordance with our Abpromise.


I look forward to your reply. Please do not hesitate to contact us if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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