• Product name

    Anti-Bim (phospho S65) antibody
    See all Bim primary antibodies
  • Description

    Rabbit polyclonal to Bim (phospho S65)
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control

    • Hek293T co-expressing rat Bim and active MEKK1



Our Abpromise guarantee covers the use of ab17935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 17 kDa).


  • Function

    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificity

    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localization

    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all


  • Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2, 5) or with
    mutant S65A Bim and MEKK1 (3, 6), were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 3% Milk-TBST buffer for one hour at room temperature, and incubated with Bim Pan antibody (1, 2, 3) or ab17935 (4, 5, 6) for two hours at room temperature in 3% Milk-TBST buffer. After washing, membranes were incubated with a goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.

    The data show that the signal is detected only in lysates co-expressing rat WT Bim and active MEKK1. This signal is abolished in cells expressing mutant rat Bim S65A and MEKK1, verifying that the signal is site-phosphospecific.

    Lysates prepared from Hek293T cells transfected with rat WT Bim alone (1, 4), cotransfected with rat WT Bim and activated MEKK1 (2


This product has been referenced in:

  • Li D  et al. Involvement of the JNK/FOXO3a/Bim Pathway in Neuronal Apoptosis after Hypoxic-Ischemic Brain Damage in Neonatal Rats. PLoS One 10:e0132998 (2015). IF ; Rat . Read more (PubMed: 26171786) »
  • Wang Y  et al. Tuberous sclerosis 1 (Tsc1)-dependent metabolic checkpoint controls development of dendritic cells. Proc Natl Acad Sci U S A 110:E4894-903 (2013). Read more (PubMed: 24282297) »
See all 3 Publications for this product

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