Anti-Bim (phospho S69) antibody (ab131477)

Rabbit polyclonal Bim (phospho S69) antibody. Validated in IHC, ICC/IF and tested in Mouse, Rat, Human. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-Bim (phospho S69) antibody
    See all Bim primary antibodies
  • Description

    Rabbit polyclonal to Bim (phospho S69)
  • Host species

    Rabbit
  • Specificity

    ab131477 detects endogenous levels of Bim only when phosphorylated at serine 69.
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, corresponding to a region around the phosphorylation site of serine 69 (

    PAS(p)PG

    ) of Human Bim (UniProt O43521).

  • Positive control

    • HeLa cells; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab131477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificity

    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similarities

    Belongs to the Bcl-2 family.
  • Domain

    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localization

    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling Bim (phospho S69) with ab131477 at 1/100 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Bim (phospho S69) with ab131477 at 1/50 dilution (left) or ab131477 preincubated with blocking peptide (right).

References

ab131477 has not yet been referenced specifically in any publications.

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