Overview

  • Product name

  • Description

    Rabbit polyclonal to BIN1
  • Host species

    Rabbit
  • Specificity

    ab137459 is expected to react with isoforms 4, 5, 6, 7, 8, 9 and 10 of Human BIN1, based on sequence homology
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-289 of Human BIN1 (UniProt ID: O00499).

  • Positive control

    • 293T, HepG2, MOLT4 and Raji whole cell lysates; A431 cells; H441 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
  • Tissue specificity

    Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
  • Involvement in disease

    Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
  • Sequence similarities

    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Phosphorylated by protein kinase C.
  • Cellular localization

    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMPH 2 antibody
    • AMPH2 antibody
    • Amphiphysin 2 antibody
    • Amphiphysin II antibody
    • Amphiphysin like protein antibody
    • amphiphysin-like antibody
    • Amphiphysin-like protein antibody
    • AMPHL antibody
    • Bin1 antibody
    • BIN1_HUMAN antibody
    • Box Dependant MYC Interacting Protein 1 antibody
    • Box-dependent myc-interacting protein 1 antibody
    • Bridging integrator 1 antibody
    • DKFZp547F068 antibody
    • MGC10367 antibody
    • MGC105358 antibody
    • Myc box dependent interacting protein 1 antibody
    • Myc box-dependent-interacting protein 1 antibody
    • SH3P9 antibody
    see all

Images

  • All lanes : Anti-BIN1 antibody (ab137459) at 1/1000 dilution

    Lane 1 : MOLT4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed A431 cells, labelling BIN1 using ab137459 at 1/200 dilution. Right panel costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded H441 xenograft tissue, labelling BIN1 using ab137459 at 1/100 dilution.

References

This product has been referenced in:

  • Ronaldson-Bouchard K  et al. Advanced maturation of human cardiac tissue grown from pluripotent stem cells. Nature 556:239-243 (2018). Read more (PubMed: 29618819) »
See 1 Publication for this product

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