Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-BIN1 antibody
See all BIN1 primary antibodies
DescriptionRabbit polyclonal to BIN1
Tested applicationsSuitable for: IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human BIN1 aa 300-350. The exact sequence is proprietary. NP_647593.1
Database link: O00499
- IP: BIN1 IP in HeLa cell lysate. IHC-P: Human prostate carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245512 was affinity purified using an epitope specific to BIN1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-15 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Tissue specificityUbiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Involvement in diseaseDefects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
modificationsPhosphorylated by protein kinase C.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for BIN1 using ab245512 at 1/1000 dilution in immunohistochemical analysis. DAB staining.
BIN1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg IP, 20% of IP loaded) using ab245512 at 10 µg/mg lysate. An alternative rabbit anti-Bin1 antibody was used for western blotting the immunoprecipitated BIN1.
Lane 1: ab245512 IP in HeLa whole cell lysate.
Lane 2: Contol IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245512 has not yet been referenced specifically in any publications.