Overview

  • Product name

  • Description

    Rabbit polyclonal to BIN1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human BIN1 aa 300-350. The exact sequence is proprietary. NP_647593.1
    Database link: O00499

  • Positive control

    • IP: BIN1 IP in HeLa cell lysate. IHC-P: Human prostate carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab245512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
    • Tissue specificity

      Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
    • Involvement in disease

      Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    • Sequence similarities

      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Phosphorylated by protein kinase C.
    • Cellular localization

      Cytoplasm and Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMPH 2 antibody
      • AMPH2 antibody
      • Amphiphysin 2 antibody
      • Amphiphysin II antibody
      • Amphiphysin like protein antibody
      • amphiphysin-like antibody
      • Amphiphysin-like protein antibody
      • AMPHL antibody
      • Bin1 antibody
      • BIN1_HUMAN antibody
      • Box Dependant MYC Interacting Protein 1 antibody
      • Box-dependent myc-interacting protein 1 antibody
      • Bridging integrator 1 antibody
      • DKFZp547F068 antibody
      • MGC10367 antibody
      • MGC105358 antibody
      • Myc box dependent interacting protein 1 antibody
      • Myc box-dependent-interacting protein 1 antibody
      • SH3P9 antibody
      see all

    Images

    • Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for BIN1 using ab245512 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

    • BIN1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg IP, 20% of IP loaded) using ab245512 at 10 µg/mg lysate. An alternative rabbit anti-Bin1 antibody was used for western blotting the immunoprecipitated BIN1.

      Lane 1: ab245512 IP in HeLa whole cell lysate.
      Lane 2: Contol IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab245512 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab245512.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up