• Product name

  • Description

    Rabbit polyclonal to BIN1
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse) - corresponding to region surrounding amino acid 578 of mouse BIN 1.

  • Positive control

    • Jurkat cells



Our Abpromise guarantee covers the use of ab27796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 65 kDa and a 45kDa fragment can be detected in apoptotic samples.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
    • Tissue specificity

      Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
    • Involvement in disease

      Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
    • Sequence similarities

      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • Post-translational

      Phosphorylated by protein kinase C.
    • Cellular localization

      Cytoplasm and Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMPH 2 antibody
      • AMPH2 antibody
      • Amphiphysin 2 antibody
      • Amphiphysin II antibody
      • Amphiphysin like protein antibody
      • amphiphysin-like antibody
      • Amphiphysin-like protein antibody
      • AMPHL antibody
      • Bin1 antibody
      • BIN1_HUMAN antibody
      • Box Dependant MYC Interacting Protein 1 antibody
      • Box-dependent myc-interacting protein 1 antibody
      • Bridging integrator 1 antibody
      • DKFZp547F068 antibody
      • MGC10367 antibody
      • MGC105358 antibody
      • Myc box dependent interacting protein 1 antibody
      • Myc box-dependent-interacting protein 1 antibody
      • SH3P9 antibody
      see all


    • All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml

      Lane 1 : Jurkat whole cell lysate (ab7899)
      Lane 2 : Jurkat cells treated with camptothecin for 6 hours

      Predicted band size: 65 kDa
      Observed band size: 64 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 48 kDa (possible cleavage fragment)


    This product has been referenced in:

    • Chapuis J  et al. Increased expression of BIN1 mediates Alzheimer genetic risk by modulating tau pathology. Mol Psychiatry N/A:N/A (2013). WB ; Human . Read more (PubMed: 23399914) »
    See 1 Publication for this product

    Customer reviews and Q&As


    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab27796 with the order number 1080280.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

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