Key features and details
- Rabbit polyclonal to BIN1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-BIN1 antibody
See all BIN1 primary antibodies
DescriptionRabbit polyclonal to BIN1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Mouse) - corresponding to region surrounding amino acid 578 of mouse BIN 1.
- Jurkat cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Tissue specificityUbiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Involvement in diseaseDefects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
modificationsPhosphorylated by protein kinase C.
Cellular localizationCytoplasm and Nucleus.
- Information by UniProt
- AMPH 2 antibody
- AMPH2 antibody
- Amphiphysin 2 antibody
All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml
Lane 1 :
Jurkat whole cell lysate (ab7899)
Lane 2 : Jurkat cells treated with camptothecin for 6 hours
Predicted band size: 65 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible cleavage fragment)
ab27796 has been referenced in 1 publication.
- Chapuis J et al. Increased expression of BIN1 mediates Alzheimer genetic risk by modulating tau pathology. Mol Psychiatry N/A:N/A (2013). WB ; Human . PubMed: 23399914