Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)
  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)

Key features and details

  • Biotin Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) - Azide free
  • Suitable for: ICC/IF
  • Reacts with: Mouse, a wide range of other species
  • Conjugation: Biotin
  • Isotype: IgM

Overview

  • Product name

    Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free
    See all 5-methylcytosine (5-mC) primary antibodies

  • Description

    Biotin Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) - Azide free

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, a wide range of other species

  • Immunogen

    Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA).

  • Positive control

    • Chlamydomonas me-1 and mouse embryonic stem cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab179914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.

Target

  • Form

    The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine. For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make the nucleotides accessible for the antibody. Denaturing methods vary depending on each application.

  • Alternative names

    • 5 m C antibody
    • 5 mC antibody
    • 5 me C antibody
    • 5 Me Cytidine antibody
    • 5 Me Cytosine antibody
    • 5 meC antibody
    • 5 Methycytosine antibody
    • 5 Methyl Cytidine antibody
    • 5 Methyl Cytosine antibody
    • 5 Methyl-Cytidine antibody
    • 5 Methyl-Cytosine antibody
    • 5 MethylCytidine antibody
    • 5 MethylCytosine antibody
    • 5-mC antibody
    • 5-Me Cytidine antibody
    • 5-Me Cytosine antibody
    • 5-meC antibody
    • 5-Methyl Cytidine antibody
    • 5-Methyl-Cytidine antibody
    • 5-Methyl-Cytosine antibody
    • 5-MethylCytidine antibody
    • 5-MethylCytosine antibody
    • 5mC antibody
    • methyl CpG antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)
    Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)

    Immunofluorescence analysis of Clamydomonas me-1 cells, labeling 5-Methyl Cytosine using ab179914 at a 1/50 dilution, followed by FITC-conjugated secondary antibody (green). DAPI was used to stain DNA (magenta). Upper panels (a, b and c) correspond to vegetative cells and lower panels (d, e and f) to gametes.

  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)
    Immunocytochemistry/ Immunofluorescence - Biotin Anti-5-methylcytosine (5-mC) antibody [5MC-CD] - Azide free (ab179914)

    Immunofluorescence analysis of mouse embryonic cells, labeling 5-Methyl Cytosine using ab179914 at a 1/50 dilution. DAPI was used to stain DNA (magenta).

References (0)

Publishing research using ab179914? Please let us know so that we can cite the reference in this datasheet.

ab179914 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab179914.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com