Key features and details
- Biotin Mouse monoclonal [33D10.D2] to 6X His tag®
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Product nameBiotin Anti-6X His tag® antibody [33D10.D2]
See all 6X His tag® primary antibodies
DescriptionBiotin Mouse monoclonal [33D10.D2] to 6X His tag®
ab106261 reacts to over-expressed proteins containing the 6X His tag® fused to either the amino or carboxy terminus of transfected or transformed cells. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
10-20 BAC molecules per Mouse Monoclonal Antibody
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab106261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 1 kDa.|
RelevanceThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Cellular localizationDepends upon the localization of the parent protein tagged with hexahistidine.
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
All lanes : Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261) at 1/5000 dilution (overnight at 4°C)
Lane 1 : Purified histidine-tagged recombinant protein at 0.1 µg
Lane 2 : E. coli cell lysate containing histidine-tagged expression construct at 0.2 µg
Lane 3 : Purified GST-tagged recombinant protein at 0.1 µg
Lane 4 : Purified FLAG-tagged recombinant protein at 0.1 µg
All lanes : Peroxidase mouse secondary antibody for 30 minutes at room temperature at 1/20000 dilution
Predicted band size: 1 kDa
Block: 5% non-fat powdered milk for 1 hour at room temperature.
ELISA analysis of ab106261 Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin).
Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins.
Coating amount: 0.15 µg per welll.
Primary antibody: ab106261 at 100 µg/mL.
Dilution series: 2-fold.
Mid-point concentration: 200 ng/mL.
Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1/10000.
Substrate: TMB ELISA Substrate.
ab106261 has not yet been referenced specifically in any publications.