Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)

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Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)
  • ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)

Key features and details

  • Biotin Mouse monoclonal [33D10.D2] to 6X His tag®
  • Suitable for: WB, ELISA
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG1

Overview

  • Product name

    Biotin Anti-6X His tag® antibody [33D10.D2]
    See all 6X His tag® primary antibodies

  • Description

    Biotin Mouse monoclonal [33D10.D2] to 6X His tag®

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Specificity

    ab106261 reacts to over-expressed proteins containing the 6X His tag® fused to either the amino or carboxy terminus of transfected or transformed cells. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

  • General notes

    10-20 BAC molecules per Mouse Monoclonal Antibody

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 1% BSA, 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    33D10.D2

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 1 kDa.
ELISA 1/200000.

Target

  • Relevance

    The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

  • Cellular localization

    Depends upon the localization of the parent protein tagged with hexahistidine.

  • Alternative names

    • 6 His epitope tag antibody
    • Hexa His tag antibody
    • HHHHHH epitope tag antibody
    • HHHHHH tag antibody
    • His tag antibody
    • Polyhistidine Tag antibody
    see all

Images

  • Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)
    Western blot - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)
    All lanes : Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261) at 1/5000 dilution (overnight at 4°C)

    Lane 1 : Purified histidine-tagged recombinant protein at 0.1 µg
    Lane 2 : E. coli cell lysate containing histidine-tagged expression construct at 0.2 µg
    Lane 3 : Purified GST-tagged recombinant protein at 0.1 µg
    Lane 4 : Purified FLAG-tagged recombinant protein at 0.1 µg

    Secondary
    All lanes : Peroxidase mouse secondary antibody for 30 minutes at room temperature at 1/20000 dilution

    Predicted band size: 1 kDa



    Block: 5% non-fat powdered milk for 1 hour at room temperature.

  • ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)
    ELISA - Biotin Anti-6X His tag® antibody [33D10.D2] (ab106261)
    ELISA analysis of ab106261 Mouse monoclonal [33D10.D2] to 6X His tag® (Biotin).
    Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified proteins.
    Coating amount: 0.15 µg per welll.
    Primary antibody: ab106261 at 100 µg/mL.
    Dilution series: 2-fold.
    Mid-point concentration: 200 ng/mL.
    Secondary antibody: Peroxidase conjugated mouse secondary antibody at 1/10000.
    Substrate: TMB ELISA Substrate.

References (0)

Publishing research using ab106261? Please let us know so that we can cite the reference in this datasheet.

ab106261 has not yet been referenced specifically in any publications.

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