Biotin Anti-6X His tag® antibody (ab27025)
Key features and details
- Biotin Rabbit polyclonal to 6X His tag®
- Suitable for: Flow Cyt, WB, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-6X His tag® antibody
See all 6X His tag® primary antibodies -
Description
Biotin Rabbit polyclonal to 6X His tag® -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab27025 reacts to recombinant proteins containing the 6X His tag®.
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Tested applications
Suitable for: Flow Cyt, WB, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
HHHHHH conjugated with KLH.
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General notes
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified using the peptide immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| WB | (2) |
1/1000 - 1/5000.
|
| ELISA |
1/2000 - 1/10000.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab200208 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/5000. |
|
ELISA
1/2000 - 1/10000. |
Target
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Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. -
Cellular localization
Depends upon the localization of the parent protein tagged with hexahistidine. -
Alternative names
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab27025 has been referenced in 8 publications.
- Dobaño C et al. Highly Sensitive and Specific Multiplex Antibody Assays To Quantify Immunoglobulins M, A, and G against SARS-CoV-2 Antigens. J Clin Microbiol 59:N/A (2021). PubMed: 33127841
- Wei Y et al. Activation of KRas-ERK1/2 signaling drives the initiation and progression of glioma by suppressing the acetylation of histone H4 at lysine 16. Life Sci 225:55-63 (2019). PubMed: 30946839
- Mitchell LA et al. PD-L1 checkpoint blockade delivered by retroviral replicating vector confers anti-tumor efficacy in murine tumor models. Oncotarget 10:2252-2269 (2019). PubMed: 31040917
- Hosokawa K et al. The telomere binding protein Pot1 maintains haematopoietic stem cell activity with age. Nat Commun 8:804 (2017). PubMed: 28986560
- Kim B et al. TUT7 controls the fate of precursor microRNAs by using three different uridylation mechanisms. EMBO J 34:1801-15 (2015). PubMed: 25979828
- Spång HC et al. Heterodimeric Barnase-Barstar Vaccine Molecules: Influence of One versus Two Targeting Units Specific for Antigen Presenting Cells. PLoS One 7:e45393 (2012). WB . PubMed: 23028981
- Lee HK et al. In vivo requirement for Atg5 in antigen presentation by dendritic cells. Immunity 32:227-39 (2010). PubMed: 20171125
- de Goeij BE et al. HER2 monoclonal antibodies that do not interfere with receptor heterodimerization-mediated signaling induce effective internalization and represent valuable components for rational antibody-drug conjugate design. MAbs 6:392-402 (0). PubMed: 24492309