Biotin Anti-alpha 2 Macroglobulin antibody (ab7338)

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Key features and details

  • Biotin Goat polyclonal to alpha 2 Macroglobulin
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-alpha 2 Macroglobulin antibody
    See all alpha 2 Macroglobulin primary antibodies

  • Description

    Biotin Goat polyclonal to alpha 2 Macroglobulin

  • Host species

    Goat

  • Conjugation

    Biotin

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Alpha-2-Macroglobulin [Human Plasma]

  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...

  • Purity

    IgG fraction

  • Purification notes

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

  • Application notes
    Immunoblotting (western or dot blot)
    ELISA (1:9,000 to 1:36,000)
    Immunohistochemistry
    Immunomicroscopy
    as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

    • Target

    • Function

      Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

    • Tissue specificity

      Secreted in plasma.

    • Sequence similarities

      Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.

    • Developmental stage

      Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • A2m antibody
        • A2MG_HUMAN antibody
        • Alpha 2 M antibody
        • Alpha 2M antibody
        • Alpha-2-M antibody
        • Alpha-2-macroglobulin antibody
        • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 antibody
        • CPAMD5 antibody
        • DKFZp779B086 antibody
        • FWP007 antibody
        • S863 7 antibody
        see all

      References (1)

      Publishing research using ab7338? Please let us know so that we can cite the reference in this datasheet.

      ab7338 has been referenced in 1 publication.

      • Jensen PE  et al. Significantly increased fractions of transformed to total alpha2-macroglobulin concentrations in plasma from patients with multiple sclerosis. Biochim Biophys Acta 1690:203-7 (2004). ELISA . PubMed: 15511627

      Customer reviews and Q&As

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      Question

      Have the epitopes been determined?

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      Answer

      Thank you for your enquiry. As these 4 antibodies are all polyclonal antibodies, each will recognize multiple epitopes. If you have any more questions, please do contact us again.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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