Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)](https://www.abcam.com/ps/products/125/ab125057/Images/ab125057-139201-anti-alpha-smooth-muscle-actin-antibody-1a4-biotin-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)")
Key features and details
- Biotin Mouse monoclonal [1A4] to alpha smooth muscle Actin
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-alpha smooth muscle Actin antibody [1A4]
See all alpha smooth muscle Actin primary antibodies -
Description
Biotin Mouse monoclonal [1A4] to alpha smooth muscle Actin -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to alpha smooth muscle Actin.
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Positive control
- In IHC, this antibody gave a positive signal in human ductal carcinoma FFPE Tissue section.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
1A4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
- Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
- HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
- Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
- Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
- FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
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IHC-P
Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
Sequence similarities
Belongs to the actin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 59 Human
- Omim: 102620 Human
- SwissProt: P62736 Human
- Unigene: 500483 Human
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Alternative names
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)IHC image of ab125057 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab125057 has been referenced in 7 publications.
- Uryga AK et al. Telomere damage promotes vascular smooth muscle cell senescence and immune cell recruitment after vessel injury. Commun Biol 4:611 (2021). PubMed: 34021256
- Chou YH et al. Methylation in pericytes after acute injury promotes chronic kidney disease. J Clin Invest 130:4845-4857 (2020). PubMed: 32749240
- Ohigashi H et al. Ocular instillation of vitamin A-coupled liposomes containing HSP47 siRNA ameliorates dry eye syndrome in chronic GVHD. Blood Adv 3:1003-1010 (2019). PubMed: 30940635
- Clement M et al. MARK4 (Microtubule Affinity-Regulating Kinase 4)-Dependent Inflammasome Activation Promotes Atherosclerosis-Brief Report. Arterioscler Thromb Vasc Biol 39:1645-1651 (2019). PubMed: 31167564
- Dobnikar L et al. Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels. Nat Commun 9:4567 (2018). PubMed: 30385745
- Liu S et al. Members of Bitter Taste Receptor ClusterTas2r143/Tas2r135/Tas2r126Are Expressed in the Epithelium of Murine Airways and Other Non-gustatory Tissues. Front Physiol 8:849 (2017). PubMed: 29163195
- Kaur H et al. Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system. Nat Commun 8:15700 (2017). IHC-Fr . PubMed: 28621310