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  1. Link

    biotin-alpha-smooth-muscle-actin-antibody-1a4-ab125057.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin
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Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)

    Key features and details

    • Biotin Mouse monoclonal [1A4] to alpha smooth muscle Actin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    Carrier Free

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    Overview

    • Product name

      Biotin Anti-alpha smooth muscle Actin antibody [1A4]
      See all alpha smooth muscle Actin primary antibodies
    • Description

      Biotin Mouse monoclonal [1A4] to alpha smooth muscle Actin
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide corresponding to alpha smooth muscle Actin.

    • Positive control

      • In IHC, this antibody gave a positive signal in human ductal carcinoma FFPE Tissue section.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      IgG fraction
    • Clonality

      Monoclonal
    • Clone number

      1A4
    • Isotype

      IgG2a
    • Light chain type

      kappa
    • Research areas

      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microfilaments
      • Actin etc
      • Actin
      • Stem Cells
      • Lineage Markers
      • Mesoderm
      • Stem Cells
      • Mesenchymal Stem Cells
      • Myogenesis
      • Cardiovascular
      • Heart
      • Contractility
      • Contractile Proteins
      • Actins
      • Cardiovascular
      • Vasculature
      • Vasculature Markers
      • Arterial
      • Cardiovascular
      • Vasculature
      • Smooth muscle cell (SMC)
      • Cytoskeleton
      • Developmental Biology
      • Lineage specification
      • Mesoderm
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Smooth Muscle Cells

    Associated products

    • Alternative Versions

      • Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
      • Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4] (ab202368)
      • HRP Anti-alpha smooth muscle Actin antibody [1A4] (ab203696)
      • Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free (ab240654)
      • Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)
      • FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)
    • Isotype control

      • Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)
    • Recombinant Protein

      • Recombinant Human alpha smooth muscle Actin protein (ab134555)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab125057 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
    • Involvement in disease

      Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
    • Sequence similarities

      Belongs to the actin family.
    • Cellular localization

      Cytoplasm > cytoskeleton.
    • Target information above from: UniProt accession P62736 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 59 Human
      • Omim: 102620 Human
      • SwissProt: P62736 Human
      • Unigene: 500483 Human
      • Alternative names

        • a actin antibody
        • AAT6 antibody
        • ACTA_HUMAN antibody
        • ACTA2 antibody
        • Actin alpha 2 smooth muscle aorta antibody
        • Actin aortic smooth muscle antibody
        • Actin, aortic smooth muscle antibody
        • ACTSA antibody
        • ACTVS antibody
        • Alpha 2 actin antibody
        • Alpha actin 2 antibody
        • Alpha cardiac actin antibody
        • alpha sma antibody
        • Alpha-actin-2 antibody
        • Cell growth inhibiting gene 46 protein antibody
        • Cell growth-inhibiting gene 46 protein antibody
        • GIG46 antibody
        • Growth inhibiting gene 46 antibody
        • MYMY5 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-alpha smooth muscle Actin antibody [1A4] (ab125057)
        IHC image of ab125057 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      Protocols

      • Mouse on Mouse staining protocol

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab125057? Please let us know so that we can cite the reference in this datasheet.

      ab125057 has been referenced in 7 publications.

      • Uryga AK  et al. Telomere damage promotes vascular smooth muscle cell senescence and immune cell recruitment after vessel injury. Commun Biol 4:611 (2021). PubMed: 34021256
      • Chou YH  et al. Methylation in pericytes after acute injury promotes chronic kidney disease. J Clin Invest 130:4845-4857 (2020). PubMed: 32749240
      • Ohigashi H  et al. Ocular instillation of vitamin A-coupled liposomes containing HSP47 siRNA ameliorates dry eye syndrome in chronic GVHD. Blood Adv 3:1003-1010 (2019). PubMed: 30940635
      • Clement M  et al. MARK4 (Microtubule Affinity-Regulating Kinase 4)-Dependent Inflammasome Activation Promotes Atherosclerosis-Brief Report. Arterioscler Thromb Vasc Biol 39:1645-1651 (2019). PubMed: 31167564
      • Dobnikar L  et al. Disease-relevant transcriptional signatures identified in individual smooth muscle cells from healthy mouse vessels. Nat Commun 9:4567 (2018). PubMed: 30385745
      • Liu S  et al. Members of Bitter Taste Receptor ClusterTas2r143/Tas2r135/Tas2r126Are Expressed in the Epithelium of Murine Airways and Other Non-gustatory Tissues. Front Physiol 8:849 (2017). PubMed: 29163195
      • Kaur H  et al. Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system. Nat Commun 8:15700 (2017). IHC-Fr . PubMed: 28621310

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Frozen sections) abreview for Anti-alpha smooth muscle Actin antibody [1A4] (Biotin)

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Bone marrow)
      Permeabilization
      Yes - 0.05% Tween20
      Specification
      Bone marrow
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Bryan Anthony

      Verified customer

      Submitted Mar 21 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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