Key features and details
- Biotin Rabbit polyclonal to alpha Tubulin
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Zebrafish
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-alpha Tubulin antibody
See all alpha Tubulin primary antibodies
DescriptionBiotin Rabbit polyclonal to alpha Tubulin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Human, Zebrafish
Synthetic peptide within Human alpha Tubulin (C terminal). The exact sequence is proprietary. 18 amino acid peptide
Database link: P68366
- WB: HEK-293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, NIH/3T3, Raji, THP-1 and U937 whole cell lysates; human brain lysate; mouse brain and lung lysates; rat brain lysate; rabbit brain lysate; chicken small intestine lysate; Zebrafish lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- Mouse lung normal tissue lysate - total protein (ab29297)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Mouse brain tissue lysate - total protein (ab30151)
- Daudi whole cell lysate (ab3951)
- Mouse brain tissue lysate - total protein (ab4022)
- NIH 3T3 whole cell lysate (ab7179)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A431 whole cell lysate (ab7909)
- A549 whole cell lysate (ab7910)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab255321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.|
FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Sequence similaritiesBelongs to the tubulin family.
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
All lanes : Biotin Anti-alpha Tubulin antibody (ab255321) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 7 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 8 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 9 : MOLT cell lysate
Lane 10 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 11 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 12 : THP-1 (human monocytic leukemia cell line) cell lysate
Lane 13 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 14 : Human brain lysate
Lane 15 : Mouse brain lysate
Lane 16 : Rat brain lysate
Lane 17 : Rabbit brain lysate
Lane 18 : Mouse lung lysate
Lane 19 : Chicken small intestine lysate
Lane 20 : Zebrafish lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab255321 has not yet been referenced specifically in any publications.