Recombinant Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)
![Western blot - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)](https://www.abcam.com/ps/products/222/ab222758/Images/ab222758-282516-ab2227582.jpg "Western blot - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM113] to alpha Tubulin - C-terminal
- Suitable for: ICC/IF, WB
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal
See all alpha Tubulin primary antibodies -
Description
Biotin Rabbit monoclonal [RM113] to alpha Tubulin - C-terminal -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
This antibody reacts with alpha tubulin, including both tubulin alpha-1A chain and tubulin alpha-1B chain. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide corresponding to Human alpha Tubulin (C terminal).
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Positive control
- ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: A431 (human epidermoid carcinoma cell line) cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM113 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab222758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200. | |
| WB | 1/1000. |
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
Images
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Western blot - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758) at 1/1000 dilution + A431 cell lysate
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![Immunocytochemistry/ Immunofluorescence - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)](https://www.abcam.com/ps/products/222/ab222758/Images/ab222758-282514-ab2227581.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Tubulin (red) using ab222758 at a 1/200 dilution in ICC/IF. Nuclei counterstained with DAPI (blue).
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![Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)](https://www.abcam.com/ps/products/222/ab222758/Images/ab222758-3-benefits-of-recombinant-antibodies.png)
Biotin Anti-alpha Tubulin antibody [RM113] - C-terminal (ab222758)
Protocols
Datasheets and documents
References (0)
ab222758 has not yet been referenced specifically in any publications.
