Biotin Anti-alpha Tubulin antibody [TU-01] (ab74696)
Key features and details
- Biotin Mouse monoclonal [TU-01] to alpha Tubulin
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-alpha Tubulin antibody [TU-01]
See all alpha Tubulin primary antibodies -
Description
Biotin Mouse monoclonal [TU-01] to alpha Tubulin -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab74696 recognizes the defined epitope (aa 65-97) on the N-terminal structural domain of alpha-tubulin. This epitope is conserved within all species. -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
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General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
TU-01 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Target
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Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
Sequence similarities
Belongs to the tubulin family. -
Post-translational
modificationsSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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Alternative names
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
References (2)
ab74696 has been referenced in 2 publications.
- Chen X et al. Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging. J Biomed Opt 21:66007 (2016). PubMed: 27281064
- Zeng Z et al. Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging. Sci Rep 5:8359 (2015). IHC-P . PubMed: 25665878
