Key features and details
- Biotin Rabbit polyclonal to Amphiregulin
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-Amphiregulin antibody
See all Amphiregulin primary antibodies
DescriptionBiotin Rabbit polyclonal to Amphiregulin
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human
- WB: Recombinant human Amphiregulin protein. sELISA: Recombinant human Amphiregulin protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
FormLyophilized:Reconstitute in sterile PBS + 0.1% BSA to 0.1 - 1.0 mg/mL.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab246313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionBifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
Sequence similaritiesBelongs to the amphiregulin family.
Contains 1 EGF-like domain.
- Information by UniProt
- A REG antibody
- Amphiregulin antibody
- Amphiregulin B antibody
To detect human Amphiregulin by Western Blot analysis, ab246313 can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human Amphiregulin, respectively.
To detect human Amphiregulin by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of ab246313 is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant human Amphiregulin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246313 has not yet been referenced specifically in any publications.