Key features and details
- Mouse monoclonal [3E6] to Biotin
- Suitable for: WB, ELISA
- Reacts with: Species independent
- Isotype: IgG2a
Product nameAnti-Biotin antibody [3E6]
See all Biotin primary antibodies
DescriptionMouse monoclonal [3E6] to Biotin
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule - biotinylated denatured Ovalbumin.
Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Primary antibody notesAnti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.
Light chain typekappa
Our Abpromise guarantee covers the use of ab36406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms.|
|ELISA||1/20000. Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin.|
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
ab36406 has not yet been referenced specifically in any publications.