Overview

  • Product name

    Anti-Biotin antibody [3E6]
    See all Biotin primary antibodies
  • Description

    Mouse monoclonal [3E6] to Biotin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule - biotinylated denatured Ovalbumin.

  • General notes


    Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.

Properties

Applications

Our Abpromise guarantee covers the use of ab36406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms.
ELISA 1/20000. Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4 antibody

References

ab36406 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry and your interest in our products.

I will have to double-check with the lab regarding glycosylation of the antibodies since I do not have this information readily at hand. I will let you know once I have received this information.

For the EasyLink kits, there is a quenching step which removes any excess label and reduces background. You can find more information about the labeling process here: https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

Additional FAQs regarding these kits can be found here: https://www.abcam.com/index.html?pageconfig=resource&rid=13156

I will email you a quote for the 4 products. Currently we are running a promotion where if you buy any primary, you can get a RabMAb for free! Just mention the code RABMAB-XBSMG on the order and the cost of the rabbit monoclonal antibody will be removed from the order.

I hope this information helps. I will be in touch again with more information regarding the antibodies you requested.

Read More
Application
Western blot
Sample
Human Recombinant protein (In Vitro Translation)
Loading amount
0.005 µg
Specification
In Vitro Translation
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted May 19 2008

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