Product nameAnti-Biotin antibody
See all Biotin primary antibodies
DescriptionRabbit polyclonal to Biotin
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, ELISA, IPmore details
Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.
Our Abpromise guarantee covers the use of ab1227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/2000.|
|ICC/IF||1/200 - 1/2000.|
|WB||1/1000 - 1/30000.|
|ELISA||1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.|
|IP||Use at an assay dependent concentration.|
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
This product has been referenced in:
- Banuelos CA et al. Sintokamide A Is a Novel Antagonist of Androgen Receptor That Uniquely Binds Activation Function-1 in Its Amino-terminal Domain. J Biol Chem 291:22231-22243 (2016). Read more (PubMed: 27576691) »
- Waaijers S et al. A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. BMC Biol 14:66 (2016). WB . Read more (PubMed: 27506200) »