- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Biotin antibody
See all Biotin primary antibodies -
DescriptionRabbit polyclonal to Biotin
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ICC/IF, WB, ELISA, IPmore details
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Immunogen
Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
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Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/2000. | |
| ICC/IF | 1/200 - 1/2000. | |
| WB | 1/1000 - 1/30000. | |
| ELISA | 1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000. | |
| IP | Use at an assay dependent concentration. |
Target
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RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
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Alternative names
- Vitamin B4 antibody
Images
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Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Protocols
References
This product has been referenced in:
- Banuelos CA et al. Sintokamide A Is a Novel Antagonist of Androgen Receptor That Uniquely Binds Activation Function-1 in Its Amino-terminal Domain. J Biol Chem 291:22231-22243 (2016). Read more (PubMed: 27576691) »
- Waaijers S et al. A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. BMC Biol 14:66 (2016). WB . Read more (PubMed: 27506200) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"