Anti-Biotin antibody (ab1227)
Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: IHC-P, ICC/IF, WB, ELISA, IP
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Biotin antibody
See all Biotin primary antibodies -
Description
Rabbit polyclonal to Biotin -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WB, ELISA, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/2000.
|
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| ICC/IF | (2) |
1/200 - 1/2000.
|
| WB | (3) |
1/1000 - 1/30000.
|
| ELISA |
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
|
|
| IP | (2) |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
1/200 - 1/2000. |
|
ICC/IF
1/200 - 1/2000. |
|
WB
1/1000 - 1/30000. |
|
ELISA
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000. |
|
IP
Use at an assay dependent concentration. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Images
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Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Protocols
References (11)
ab1227 has been referenced in 11 publications.
- Villella VR et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). PubMed: 30498130
- Fontana GA et al. Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane. Nat Commun 10:2535 (2019). PubMed: 31182712
- Esposito S et al. Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease. Aging (Albany NY) 11:2003-2019 (2019). PubMed: 30981209
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Bollong MJ et al. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature 562:600-604 (2018). PubMed: 30323285
