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    biotin-antibody-ab1227.pdf

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Anti-Biotin antibody (ab1227)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (11)

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Abpromise

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Find out more.

Western blot - Anti-Biotin antibody (ab1227)

    Key features and details

    • Rabbit polyclonal to Biotin
    • Suitable for: IHC-P, ICC/IF, WB, ELISA, IP
    • Reacts with: Species independent
    • Isotype: IgG

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    Overview

    • Product name

      Anti-Biotin antibody
      See all Biotin primary antibodies
    • Description

      Rabbit polyclonal to Biotin
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-P, ICC/IF, WB, ELISA, IPmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 6.8
      Preservative: 0.1% Sodium azide
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Epitope Tags
      • Conjugates
      • Signal Transduction
      • Metabolism
      • Vitamins / Minerals
      • Metabolism
      • Pathways and Processes
      • Cofactors, Vitamins / minerals
      • Vitamins / minerals
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1227 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/200 - 1/2000.
    ICC/IF (2)
    1/200 - 1/2000.
    WB (3)
    1/1000 - 1/30000.
    ELISA
    1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
    IP (2)
    Use at an assay dependent concentration.
    Notes
    IHC-P
    1/200 - 1/2000.
    ICC/IF
    1/200 - 1/2000.
    WB
    1/1000 - 1/30000.
    ELISA
    1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
    IP
    Use at an assay dependent concentration.

    Target

    • Relevance

      Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • Alternative names

      • Vitamin B4 antibody

    Images

    • Western blot - Anti-Biotin antibody (ab1227)
      Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John Andersen
      Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.

      Secondary
      Streptavidin-HRP

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 10 seconds

      See Abreview

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (11)

    Publishing research using ab1227? Please let us know so that we can cite the reference in this datasheet.

    ab1227 has been referenced in 11 publications.

    • Villella VR  et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). PubMed: 30498130
    • Fontana GA  et al. Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane. Nat Commun 10:2535 (2019). PubMed: 31182712
    • Esposito S  et al. Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease. Aging (Albany NY) 11:2003-2019 (2019). PubMed: 30981209
    • Wang J  et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
    • Bollong MJ  et al. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature 562:600-604 (2018). PubMed: 30323285
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Biotin antibody

    Below Average
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (293 cells)
    Specification
    293 cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 30 2012

    Immunoprecipitation abreview for Anti-Biotin antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - nuclear (mouse liver)
    Total protein in input
    50 µg
    Specification
    mouse liver
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 30 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Biotin antibody

    Below Average
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (mouse ES cells)
    Specification
    mouse ES cells
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 30 2012

    Western blot abreview for Anti-Biotin antibody

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (mouse liver)
    Loading amount
    50 µg
    Specification
    mouse liver
    Gel Running Conditions
    Reduced Denaturing (8% Precast Gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 23 2012

    Immunoprecipitation abreview for Anti-Biotin antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - nuclear (HEK293)
    Total protein in input
    2000 µg
    Specification
    HEK293
    Immuno-precipitation step
    Other - Protein G Dynabeads
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2011

    Western blot abreview for Anti-Biotin antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HEK293-T)
    Loading amount
    100 µg
    Specification
    HEK293-T
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 06 2011

    Western blot abreview for Anti-Biotin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Ovarian tissue)
    Loading amount
    50 µg
    Specification
    Ovarian tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Mr. John Andersen

    Verified customer

    Submitted Sep 13 2006

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