Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: WB, Competitive ELISA, IP, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Biotin antibody
See all Biotin primary antibodies
DescriptionRabbit polyclonal to Biotin
SpecificityReacts with free biotin and biotinylated antibody and proteins
Tested applicationsSuitable for: WB, Competitive ELISA, IP, ICC/IFmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.00
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified with d-Biotin-agarose beads
Our Abpromise guarantee covers the use of ab53494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.|
|Competitive ELISA||Use at an assay dependent dilution. (sensitivity: 0.01 ng/mL).|
|IP||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
RWP1 cells were lysed with Polysomal Lysis
Buffer and diluted in binding buffer (20 mM Hepes
pH 7.6, 150 mM KCl, 3 mM MgCl2, 10% glycerol, 3 mg/ml BSA, 0.2 Triton X-100, 20 µg/ml poly dI-dC, 1 mM DDT, plus protease and RNase inhibitors). Pre-clearing was performed by incubating with protein G-agarose blocked with salmon sperm (1 mg/mL) and mouse IgG (10 µg/mL). After pre-clearing, samples (500 µg) were incubated 4 hours in binding buffer with the DNA-biotinylated probes (2 µg) and antibody against biotin (20 µg/mL) in a final volume of 1 mL. Samples were pulled-down with protein G-agarose, washed with binding buffer supplemented with 0.1%
Tween-20 and analyzed by Western Blot for Suz12.
ab53494 has been referenced in 22 publications.
- Hao W et al. Enzymatically inactive OGG1 binds to DNA and steers base excision repair toward gene transcription. FASEB J 34:7427-7441 (2020). PubMed: 32378256
- Leimbacher PA et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438
- Galbiati A & d'Adda di Fagagna F DNA Damage In Situ Ligation Followed by Proximity Ligation Assay (DI-PLA). Methods Mol Biol 1896:11-20 (2019). PubMed: 30474835
- Chen C et al. RNA polymerase II-independent recruitment of SPT6L at transcription start sites in Arabidopsis. Nucleic Acids Res 47:6714-6725 (2019). PubMed: 31127286
- Mukherjee A et al. Understanding How Wnt Influences Destruction Complex Activity and ß-Catenin Dynamics. iScience 6:13-21 (2018). PubMed: 30240607