Product nameAnti-Biotin antibody
See all Biotin primary antibodies
DescriptionGoat polyclonal to Biotin
Tested applicationsSuitable for: Conjugation, ELISA, Immunodiffusion, IPmore details
Species reactivityReacts with: Species independent
Full length protein conjugated to KLH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
Our Abpromise guarantee covers the use of ab6643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent dilution.|
|ELISA||1/20000 - 1/50000.|
|Immunodiffusion||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
This product has been referenced in:
- Andresini O et al. The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region. Epigenetics Chromatin 12:8 (2019). Read more (PubMed: 30651140) »
- Boucher D et al. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity. J Exp Med 215:827-840 (2018). Read more (PubMed: 29432122) »