Anti-Biotin antibody (ab6643)

Submit a review Q&A (6)References (7)

Key features and details

  • Goat polyclonal to Biotin
  • Suitable for: ELISA, Immunodiffusion, IP
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-Biotin antibody
    See all Biotin primary antibodies

  • Description

    Goat polyclonal to Biotin

  • Host species

    Goat

  • Tested applications

    Suitable for: ELISA, Immunodiffusion, IPmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20000 - 1/50000.
Immunodiffusion
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
ELISA
1/20000 - 1/50000.
Immunodiffusion
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • Alternative names

    • Vitamin B4 antibody

References (7)

Publishing research using ab6643? Please let us know so that we can cite the reference in this datasheet.

ab6643 has been referenced in 7 publications.

  • Andresini O  et al. The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region. Epigenetics Chromatin 12:8 (2019). PubMed: 30651140
  • Boucher D  et al. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity. J Exp Med 215:827-840 (2018). PubMed: 29432122
  • Omeragic A  et al. Peroxisome proliferator-activated receptor-gamma: potential molecular therapeutic target for HIV-1-associated brain inflammation. J Neuroinflammation 14:183 (2017). PubMed: 28886715
  • Wu RA  et al. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes. Elife 4:N/A (2015). WB . PubMed: 26457608
  • Leduc F  et al. Genome-wide mapping of DNA strand breaks. PLoS One 6:e17353 (2011). IP . PubMed: 21364894
  • Best JM  et al. Small GTPase Rab11b regulates degradation of surface membrane L-type Cav1.2 channels. Am J Physiol Cell Physiol 300:C1023-33 (2011). WB . PubMed: 21248079
  • Mariner PD  et al. Human Alu RNA is a modular transacting repressor of mRNA transcription during heat shock. Mol Cell 29:499-509 (2008). PubMed: 18313387

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

Thank you very much for your answer. But, would you recomend me anyone in special, because I do not know which are the differences among them. I would appreciate a lot if you can tell me the differences among them. How many days do you need to make the antibody arriving me? Thank you again,

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Answer

I have looked in detail at the datasheets for all three antibodies and the differences are: 1) the species in which they are raised in: -ab6643 and ab34579 are raised in goat -ab34552 and ab34826 are raised in rabbit 2) the way the antibodies were purified. -ab6643 was purified using biotin coupled to sepharose beads (high quality purification) -ab34552 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer -ab34579 was purified from monospecific antiserum by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -ab34826 was purified from monoserum delipidified and defibrinated. This will affect the amount of antibody supplied per vial (so for the same price of €330 per vial we sell 0.1mg of ab6643 (the highly purified product) or 10 mg of ab34579 (less purified version). All antibodies except ab6643 are not in stock and will take 1-2 weeks to arrive following your order. Ab6643 is in stock and will take 1-2 days to arrive. I hope this information will help you decide which product is most suitable for your needs,

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Question

I need an anti-biotin antibody for immunoprecipitation with protein A or G magnetic microbeads (Miltenyi Biotec). Any of the following would work ok for it?: ab6643, ab34552, ab34579, ab34826.

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Answer

Thank you for your enquiry. None of the antibodies ab6643, ab34552, ab34579 and ab34826 have been tested in IP with magnetic microbeads so I cannot guarantee they will work with those beads. HOwever I can confirm that they work with protein A and G sepharose beads. If in your experience these beads work in similar ways we can predict they may work in your beads. I'm sorry I couldn't help you more on this occasion,

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Question

How was this antibody tested in IHC-P? What was the dilution used, and for how long and at what temperature was it incubated?

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Answer

Thank you for your enquiry. I apologize, but it appears that there was a typo on this datasheet. The antibody has not been tested in IHC. I am very sorry for the inconvenience. I have updated the datasheet to reflect the correct information. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

What is the pI of anti-biotin?

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Answer

This is an IgG antibody. We have never done any isoelectric point studies ourselves, but according to a website I have found, the isoelectric point of IgG is pH 6.1 - 8.5. I hope this is useful!

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Question

Do you know if this antibody can be used as a marker for mitochondria in immunofluorescence assays?

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Answer

I am unsure why you would wish to use this antibody (Goat polyclonal to Biotin) for this purpose. Do you have a biotin-labelled protein that localises specifically to the mitochondrion? You would have to use an anti-goat secondary conjugated to a fluorescent tag.

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Question

what is the biotin binding capacity of this affinity purified Ab?

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Answer

Sorry, we have no data on the biotin binding capacity.

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