For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    biotin-antibody-ab6643.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Epitope Tags Conjugates
Share by email

Anti-Biotin antibody (ab6643)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Goat polyclonal to Biotin
  • Suitable for: ELISA, Immunodiffusion, IP
  • Reacts with: Species independent
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-FITC antibody [F4/1] (ab112511)
Conjugation
Product image
Gold Conjugation Kit (20 nm, 20 OD) (ab188215)
Primary
Product image
Anti-Progesterone antibody [EPPTX-R1] (ab215527)

View more associated products

Overview

  • Product name

    Anti-Biotin antibody
    See all Biotin primary antibodies
  • Description

    Goat polyclonal to Biotin
  • Host species

    Goat
  • Tested applications

    Suitable for: ELISA, Immunodiffusion, IPmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length protein conjugated to KLH.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
1/20000 - 1/50000.
Immunodiffusion
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
ELISA
1/20000 - 1/50000.
Immunodiffusion
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4 antibody

Protocols

  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (7)

    Publishing research using ab6643? Please let us know so that we can cite the reference in this datasheet.

    ab6643 has been referenced in 7 publications.

    • Andresini O  et al. The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region. Epigenetics Chromatin 12:8 (2019). PubMed: 30651140
    • Boucher D  et al. Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity. J Exp Med 215:827-840 (2018). PubMed: 29432122
    • Omeragic A  et al. Peroxisome proliferator-activated receptor-gamma: potential molecular therapeutic target for HIV-1-associated brain inflammation. J Neuroinflammation 14:183 (2017). PubMed: 28886715
    • Wu RA  et al. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes. Elife 4:N/A (2015). WB . PubMed: 26457608
    • Leduc F  et al. Genome-wide mapping of DNA strand breaks. PLoS One 6:e17353 (2011). IP . PubMed: 21364894
    • Best JM  et al. Small GTPase Rab11b regulates degradation of surface membrane L-type Cav1.2 channels. Am J Physiol Cell Physiol 300:C1023-33 (2011). WB . PubMed: 21248079
    • Mariner PD  et al. Human Alu RNA is a modular transacting repressor of mRNA transcription during heat shock. Mol Cell 29:499-509 (2008). PubMed: 18313387

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    Thank you very much for your answer. But, would you recomend me anyone in special, because I do not know which are the differences among them. I would appreciate a lot if you can tell me the differences among them. How many days do you need to make the antibody arriving me? Thank you again,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 17 2006

    Answer

    I have looked in detail at the datasheets for all three antibodies and the differences are: 1) the species in which they are raised in: -ab6643 and ab34579 are raised in goat -ab34552 and ab34826 are raised in rabbit 2) the way the antibodies were purified. -ab6643 was purified using biotin coupled to sepharose beads (high quality purification) -ab34552 was purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer -ab34579 was purified from monospecific antiserum by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -ab34826 was purified from monoserum delipidified and defibrinated. This will affect the amount of antibody supplied per vial (so for the same price of €330 per vial we sell 0.1mg of ab6643 (the highly purified product) or 10 mg of ab34579 (less purified version). All antibodies except ab6643 are not in stock and will take 1-2 weeks to arrive following your order. Ab6643 is in stock and will take 1-2 days to arrive. I hope this information will help you decide which product is most suitable for your needs,

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2006

    Question

    I need an anti-biotin antibody for immunoprecipitation with protein A or G magnetic microbeads (Miltenyi Biotec). Any of the following would work ok for it?: ab6643, ab34552, ab34579, ab34826.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 14 2006

    Answer

    Thank you for your enquiry. None of the antibodies ab6643, ab34552, ab34579 and ab34826 have been tested in IP with magnetic microbeads so I cannot guarantee they will work with those beads. HOwever I can confirm that they work with protein A and G sepharose beads. If in your experience these beads work in similar ways we can predict they may work in your beads. I'm sorry I couldn't help you more on this occasion,

    Read More

    Abcam Scientific Support

    Answered on Jul 17 2006

    Question

    How was this antibody tested in IHC-P? What was the dilution used, and for how long and at what temperature was it incubated?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2006

    Answer

    Thank you for your enquiry. I apologize, but it appears that there was a typo on this datasheet. The antibody has not been tested in IHC. I am very sorry for the inconvenience. I have updated the datasheet to reflect the correct information. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 28 2006

    Question

    What is the pI of anti-biotin?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2002

    Answer

    This is an IgG antibody. We have never done any isoelectric point studies ourselves, but according to a website I have found, the isoelectric point of IgG is pH 6.1 - 8.5. I hope this is useful!

    Read More

    Abcam Scientific Support

    Answered on Jun 20 2002

    Question

    Do you know if this antibody can be used as a marker for mitochondria in immunofluorescence assays?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 28 2001

    Answer

    I am unsure why you would wish to use this antibody (Goat polyclonal to Biotin) for this purpose. Do you have a biotin-labelled protein that localises specifically to the mitochondrion? You would have to use an anti-goat secondary conjugated to a fluorescent tag.

    Read More

    Abcam Scientific Support

    Answered on Jan 03 2002

    Question

    what is the biotin binding capacity of this affinity purified Ab?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 13 2001

    Answer

    Sorry, we have no data on the biotin binding capacity.

    Read More

    Abcam Scientific Support

    Answered on Aug 14 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.