Overview

  • Product name
    Anti-Biotin antibody
    See all Biotin primary antibodies
  • Description
    Goat polyclonal to Biotin
  • Host species
    Goat
  • Tested applications
    Suitable for: Conjugation, ELISA, Immunodiffusion, IPmore details
  • Immunogen

    Full length protein conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab6643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ELISA 1/20000 - 1/50000.
Immunodiffusion Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • Relevance
    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References

This product has been referenced in:
  • Omeragic A  et al. Peroxisome proliferator-activated receptor-gamma: potential molecular therapeutic target for HIV-1-associated brain inflammation. J Neuroinflammation 14:183 (2017). Read more (PubMed: 28886715) »
  • Wu RA  et al. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes. Elife 4:N/A (2015). WB . Read more (PubMed: 26457608) »

See all 5 Publications for this product

Publishing research using ab6643? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Thank you very much for your answer. But, would you recomend me anyone in special, because I do not know which are the differences among them. I would appreciate a lot if you can tell me the differences among them. How many days do you need to...

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I have looked in detail at the datasheets for all three antibodies and the differences are: 1) the species in which they are raised in: -ab6643 and ab34579 are raised in goat -ab34552 and ab34826 are raised in rabbit 2) the way the antibodies ...

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I need an anti-biotin antibody for immunoprecipitation with protein A or G magnetic microbeads (Miltenyi Biotec). Any of the following would work ok for it?: ab6643, ab34552, ab34579, ab34826.

Thank you for your enquiry. None of the antibodies ab6643, ab34552, ab34579 and ab34826 have been tested in IP with magnetic microbeads so I cannot guarantee they will work with those beads. HOwever I can confirm that they work with protein A and G s...

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How was this antibody tested in IHC-P? What was the dilution used, and for how long and at what temperature was it incubated?

Thank you for your enquiry. I apologize, but it appears that there was a typo on this datasheet. The antibody has not been tested in IHC. I am very sorry for the inconvenience. I have updated the datasheet to reflect the correct information. I...

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What is the pI of anti-biotin?

This is an IgG antibody. We have never done any isoelectric point studies ourselves, but according to a website I have found, the isoelectric point of IgG is pH 6.1 - 8.5. I hope this is useful!

Do you know if this antibody can be used as a marker for mitochondria in immunofluorescence assays?

I am unsure why you would wish to use this antibody (Goat polyclonal to Biotin) for this purpose. Do you have a biotin-labelled protein that localises specifically to the mitochondrion? You would have to use an anti-goat secondary conjugated to a fluor...

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what is the biotin binding capacity of this affinity purified Ab?

Sorry, we have no data on the biotin binding capacity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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