Alkaline Phosphatase Anti-Biotin antibody (ab106217)
Key features and details
- Alkaline Phosphatase Rabbit polyclonal to Biotin
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Species independent
- Conjugation: Alkaline Phosphatase
- Isotype: IgG
Overview
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Product name
Alkaline Phosphatase Anti-Biotin antibody
See all Biotin primary antibodies -
Description
Alkaline Phosphatase Rabbit polyclonal to Biotin -
Host species
Rabbit -
Conjugation
Alkaline Phosphatase -
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and Biotinylated IgG. -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
KLH conjugated Biotin
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General notes
Dilute only prior to immediate use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.01% Magnesium chloride, 0.001% Zinc chloride, 50% Glycerol (glycerin, glycerine), 0.79% Tris HCl -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab106217 was purified from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2500.
|
|
ELISA |
1/20000.
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|
IHC-P |
1/200 - 1/1000.
|
Notes |
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WB
1/500 - 1/2500. |
ELISA
1/20000. |
IHC-P
1/200 - 1/1000. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106217 has not yet been referenced specifically in any publications.