Recombinant

Recombinant Anti-Biotin antibody [BTN/2032R] (ab234284)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BTN/2032R] to Biotin
  • Suitable for: IHC-P

Overview

  • Product name

    Anti-Biotin antibody [BTN/2032R]
    See all Biotin primary antibodies

  • Description

    Rabbit monoclonal [BTN/2032R] to Biotin

  • Host species

    Rabbit

  • Specificity

    ab234284 recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds.

  • Tested applications

    Suitable for: IHC-Pmore details

  • Immunogen

    Other Immunogen Type corresponding to Biotin. (Biotinylated sheep immunoglobulin).

  • Positive control

    • IHC-P: Human tonsil tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab234284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.

(Primary incubation for 30 minutes at room temperature).

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

  • Alternative names

    • Vitamin B4 antibody

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [BTN/2032R] (ab234284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [BTN/2032R] (ab234284)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained with biotinylated Kappa Light Chain probe and then for Biotin using ab234284 at 1 μg/ml in immunohistochemical analysis.

  • Anti-Biotin antibody [BTN/2032R] (ab234284)
    Anti-Biotin antibody [BTN/2032R] (ab234284)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab234284? Please let us know so that we can cite the reference in this datasheet.

ab234284 has not yet been referenced specifically in any publications.

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