Recombinant

Recombinant Anti-Biotin antibody [BTN/2032R] (ab234284)

Overview

  • Product name

    Anti-Biotin antibody [BTN/2032R]
    See all Biotin primary antibodies
  • Description

    Rabbit monoclonal [BTN/2032R] to Biotin
  • Host species

    Rabbit
  • Specificity

    ab234284 recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Immunogen

    Other Immunogen Type corresponding to Biotin. (Biotinylated sheep immunoglobulin).

  • Positive control

    • IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab234284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.

(Primary incubation for 30 minutes at room temperature).

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4 antibody

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained with biotinylated Kappa Light Chain probe and then for Biotin using ab234284 at 1 μg/ml in immunohistochemical analysis.

References

ab234284 has not yet been referenced specifically in any publications.

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