FITC Anti-Biotin antibody (ab53469)
Key features and details
- FITC Rabbit polyclonal to Biotin
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Overview
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Product name
FITC Anti-Biotin antibody
See all Biotin primary antibodies -
Description
FITC Rabbit polyclonal to Biotin -
Host species
Rabbit -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
d-Biotin conjugated to KLH
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General notes
Fluorescein isothionate; Ab/FITC ratio=approximately 1/20.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:1. Centrifuge the tube at 5000 rpm for 30 seconds to deposit the philosophized powder to the tube bottom; 2. Add 100 uL of distilled water and shake gently for 10 minutes until the powder is completely dissolved; 3. Add 900 uL of 50% glycerol (in distilled water) and shake gently for 5 minutes until all liquid is completely mixed. -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
1/100.
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Notes |
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ICC/IF
1/100. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Images
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ab53469 staining Biotin in human ovarian cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton ×100 in PBS and blocking with 5% serum for 30min at 230C was performed. Samples were incubated with primary antibody (1/100) for 1 hour at 23°C.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab53469 has been referenced in 4 publications.
- Dziasek K et al. Hybrid seed incompatibility in Capsella is connected to chromatin condensation defects in the endosperm. PLoS Genet 17:e1009370 (2021). PubMed: 33571184
- Rubio K et al. Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nat Commun 10:2229 (2019). PubMed: 31110176
- Ye Q et al. Frenolicin B Targets Peroxiredoxin 1 and Glutaredoxin 3 to Trigger ROS/4E-BP1-Mediated Antitumor Effects. Cell Chem Biol 26:366-377.e12 (2019). PubMed: 30661989
- Yamamoto-Fukuda T et al. Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma. J Assoc Res Otolaryngol 19:223-241 (2018). PubMed: 29549594