Key features and details
- Rabbit polyclonal to Biotin (FITC)
- Suitable for: ICC/IF
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Biotin antibody (FITC)
See all Biotin primary antibodies
DescriptionRabbit polyclonal to Biotin (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Species independent
d-Biotin conjugated to KLH
Fluorescein isothionate; Ab/FITC ratio=approximately 1/20.
FormLyophilized:1. Centrifuge the tube at 5000 rpm for 30 seconds to deposit the philosophized powder to the tube bottom; 2. Add 100 uL of distilled water and shake gently for 10 minutes until the powder is completely dissolved; 3. Add 900 uL of 50% glycerol (in distilled water) and shake gently for 5 minutes until all liquid is completely mixed.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab53469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
ab53469 staining Biotin in human ovarian cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton ×100 in PBS and blocking with 5% serum for 30min at 230C was performed. Samples were incubated with primary antibody (1/100) for 1 hour at 23°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab53469 has been referenced in 2 publications.
- Rubio K et al. Inactivation of nuclear histone deacetylases by EP300 disrupts the MiCEE complex in idiopathic pulmonary fibrosis. Nat Commun 10:2229 (2019). PubMed: 31110176
- Yamamoto-Fukuda T et al. Keratinocyte Growth Factor (KGF) Modulates Epidermal Progenitor Cell Kinetics through Activation of p63 in Middle Ear Cholesteatoma. J Assoc Res Otolaryngol 19:223-241 (2018). PubMed: 29549594